Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization, College of Landscape Architecture and Art, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
College of Life Sciences, Fujian Normal University, Fuzhou 350117, China.
Int J Mol Sci. 2023 Oct 5;24(19):14904. doi: 10.3390/ijms241914904.
WRKY is one of the largest transcription factor families in plants and plays an important role in plant growth and development as well as in abiotic and biological stresses. However, there is little information about the WRKY family in . In this study, 126 WRKY members were identified in . According to phylogenetic analysis, they were divided into three major groups, and group II was further divided into five subgroups. genes were unevenly distributed on 12 chromosomes. Additionally, the gene structure and sequence composition were similar within the same group and differed between groups, suggesting their functional diversity. The promoter sequence analysis identified a number of cis-acting elements related to plant growth and development, stress response, and secondary metabolite synthesis in the WRKY gene family. The collinearity analysis showed that gene replication events were the main driving force of gene evolution. The transcriptome data and RT-qPCR analysis suggested that genes had higher expression in the roots and ripe fruit of . In short, this paper lays a foundation for further study of the functions and molecular mechanism of WRKY gene family.
WRKY 是植物中最大的转录因子家族之一，在植物生长发育以及非生物和生物胁迫中发挥重要作用。然而，关于 WRKY 家族在 中的信息却很少。本研究在 中鉴定出 126 个 WRKY 成员。根据系统发育分析，它们被分为三大组，第二组进一步分为五个亚组。 WRKY 基因在 12 条染色体上不均匀分布。此外，同一组内的基因结构和序列组成相似，而不同组之间则不同，表明它们具有功能多样性。启动子序列分析鉴定出 WRKY 基因家族中与植物生长发育、应激反应和次生代谢物合成相关的一些顺式作用元件。共线性分析表明，基因复制事件是 基因进化的主要驱动力。转录组数据和 RT-qPCR 分析表明， WRKY 基因在 的根和成熟果实中表达水平较高。总之，本文为进一步研究 WRKY 基因家族的功能和分子机制奠定了基础。
