Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture and Art, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Int J Mol Sci. 2023 Feb 20;24(4):4174. doi: 10.3390/ijms24044174.
The YABBY gene family plays an important role in plant growth and development, such as response to abiotic stress and lateral organ development. TFs are well studied in numerous plant species, but no study has performed a genome-wide investigation of the YABBY gene family in . Therefore, a genome-wide comparative analysis of the YABBY gene family was performed to study their sequence structures, cis-acting elements, phylogenetics, expression, chromosome locations, collinearity analysis, protein interaction, and subcellular localization analysis. A total of nine genes were found, and they were further divided into four subgroups based on the phylogenetic tree. The genes in the same clade of phylogenetic tree had the same structure. The cis-element analysis showed that genes were involved in various biological processes, such as cell cycle regulation, meristem expression, responses to low temperature, and hormone signaling. were unevenly distributed on chromosomes. The transcriptomic data and real-time reverse transcription quantitative PCR (RT-qPCR) expression pattern analyses showed that genes were involved in organ development and differentiation of , and some in the subfamily may have function differentiation. The RT-qPCR analysis showed high expression of flower bud and medium flower. Moreover, all were localized in the nucleus. Therefore, this study provides a theoretical basis for the functional analysis of YABBY genes in .
YABBY 基因家族在植物生长发育中发挥着重要作用，例如应对非生物胁迫和侧生器官发育。TF 在众多植物物种中得到了广泛研究，但尚未有研究对 中的 YABBY 基因家族进行全基因组调查。因此，本研究对 YABBY 基因家族进行了全基因组比较分析，以研究其序列结构、顺式作用元件、系统发育、表达、染色体定位、共线性分析、蛋白质相互作用和亚细胞定位分析。共发现了 9 个 基因，并根据系统发育树进一步分为四个亚组。系统发育树中同一分支的基因具有相同的结构。顺式元件分析表明， 基因参与了多种生物学过程，如细胞周期调控、分生组织表达、低温响应和激素信号转导。 不均匀地分布在染色体上。转录组数据和实时荧光定量 PCR（RT-qPCR）表达模式分析表明， 基因参与了 的器官发育和分化，亚家族中的一些 可能具有功能分化。RT-qPCR 分析显示，花和中花的表达量较高。此外，所有 都定位于细胞核中。因此，本研究为 中的 YABBY 基因的功能分析提供了理论依据。
