Liu Jun, Wang Xinyu, Chen Yulong, Liu Yan, Wu Yaosong, Ren Shanshan, Li Long
Academy of Chinese Medicine, Henan University of Chinese Medicine, Zhengzhou, Henna 450046, China.
Key Laboratory of Medical Electrophysiology, Ministry of Education & Medical Electrophysiological Key Laboratory of Sichuan Province, Institute of Cardiovascular Research, Southwest Medical University, Luzhou, Sichuan 646000, China.
Genomics. 2021 Sep;113(5):3294-3309. doi: 10.1016/j.ygeno.2021.05.011. Epub 2021 May 20.
The WRKY transcription factors is one of the largest families of transcription factors (TFs) in plants and involved in multiple biological processes. However, the role of the WRKY family had not been reported in Eucommia ulmoides. In this study, 45 WRKY genes (EuWRKY1-45) with conserved WRKY domain were identified in E. ulmoides and classified into three groups. The group II was further divided into five subgroups based on phylogenetic analysis, and each clade was well supported by the conserved motifs. All the genes were located on 34 different scaffolds respectively. A number of development-, light-, hormone-, and stress-related elements were randomly distributed in the promoter sequences of EuWRKYs. Expression profiles indicated that EuWRKY genes were involved in leaf development, and majority of EuWRKYs genes were highly expressed in leaf buds. Co-expression analysis of WRKYs suggested an intricate interplay of growth-related responses. EuWRKY4 was involved in a complex proteins interaction network. Collectively, our results provide extensive insights into the WRKY gene family, thereby contributing to the screening of additional candidate genes in E. ulmoides.
WRKY转录因子是植物中最大的转录因子家族之一，参与多种生物学过程。然而，WRKY家族在杜仲中的作用尚未见报道。本研究在杜仲中鉴定出45个具有保守WRKY结构域的WRKY基因（EuWRKY1 - 45），并将其分为三组。基于系统发育分析，第二组进一步分为五个亚组，每个分支都得到保守基序的有力支持。所有基因分别位于34个不同的支架上。许多与发育、光、激素和胁迫相关的元件随机分布在EuWRKYs的启动子序列中。表达谱表明EuWRKY基因参与叶片发育，且大多数EuWRKYs基因在叶芽中高表达。WRKYs的共表达分析表明生长相关反应之间存在复杂的相互作用。EuWRKY4参与一个复杂的蛋白质相互作用网络。总体而言，我们的结果为WRKY基因家族提供了广泛的见解，从而有助于杜仲中其他候选基因的筛选。
