College of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, China; Key Laboratory of Marine Fishery Resources Exploitment & Utilization of Zhejiang Province, Hangzhou 310014, China; National R&D Branch Center for Pelagic Aquatic Products Processing (Hangzhou), Hangzhou 310014, China; Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, China.
College of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, China; Key Laboratory of Marine Fishery Resources Exploitment & Utilization of Zhejiang Province, Hangzhou 310014, China; National R&D Branch Center for Pelagic Aquatic Products Processing (Hangzhou), Hangzhou 310014, China.
Food Res Int. 2023 Jul;169:112933. doi: 10.1016/j.foodres.2023.112933. Epub 2023 May 4.
The effect of low voltage electrostatic field combined with partial freezing (LVEF- PF) treatment on storage quality and microbial community of large yellow croaker was studied. Three different methods including chilled (C), partial freezing (PF) and 6 kV/m electrostatic field combined partial freezing storage were used to preserve large yellow croaker for 18 days. Total viable counts (TVC), sensory evaluation, and physiochemical index including pH, total volatile basic nitrogen (TVB-N), K value and centrifugal loss were examined. During storage, the large yellow croaker was susceptible to microbial growth and spoilage. However, LVEF-PF treatment was found to be effective in enhancing sensory quality, inhibiting microbial growth, and maintaining myofibril microstructure. Low field nuclear magnetic resonance showed that LVEF-PF treatment reduced the migration of immobilized water to free water. At 18th day, the TVC value of LVEF-PF, PF and chilled group were 3.56 log CFU/g, 5.11 log CFU/g, 7.73 log CFU/g, respectively. Therefore, from the results of TVB-N and TVC value, the shelf life of LVEF-PF group was at least 3 days longer than PF group, and 6 days longer than the chilled group. High-throughput sequencing showed that the microbial community diversity significantly decreased during storage. The predominant bacteria in chilled, PF, LVEF-PF group at 18th day were Pseudomonas, Psychrobacter and Shewanella, respectively, and the relative abundance of spoilage bacteria such as Pseudomonas and Psychrobacter were reduced by LVEF-PF treatment, that corresponding with lower values of TVB-N and TVC value. LVEF-PF treatment could be used as a new processing and storage method to delay deterioration and prolong shelf life of large yellow croaker.
研究了低压静电场结合部分冻结（LVEF-PF）处理对大黄鱼贮藏品质和微生物群落的影响。采用冷藏（C）、部分冻结（PF）和 6 kV/m 静电场结合部分冻结贮藏三种不同方法保存大黄鱼 18 天。检测了总活菌数（TVC）、感官评价和 pH 值、总挥发性碱性氮（TVB-N）、K 值和离心损失等理化指标。贮藏过程中，大黄鱼易受微生物生长和腐败的影响。然而，LVEF-PF 处理被发现能有效提高感官品质，抑制微生物生长，保持肌原纤维微观结构。低场核磁共振显示，LVEF-PF 处理减少了固定水向自由水的迁移。第 18 天时，LVEF-PF、PF 和冷藏组的 TVC 值分别为 3.56 log CFU/g、5.11 log CFU/g、7.73 log CFU/g。因此，从 TVB-N 和 TVC 值的结果来看，LVEF-PF 组的货架期至少比 PF 组长 3 天，比冷藏组长 6 天。高通量测序结果表明，贮藏过程中微生物群落多样性显著下降。冷藏、PF、LVEF-PF 组第 18 天的优势菌分别为假单胞菌、嗜冷菌和希瓦氏菌，LVEF-PF 处理降低了腐败菌如假单胞菌和嗜冷菌的相对丰度，这与 TVB-N 和 TVC 值较低相对应。LVEF-PF 处理可作为一种新的加工和贮藏方法，延缓大黄鱼的恶化，延长货架期。
