Ganjiang Chinese Medicine Innovation Center, Nanchang 330100, China.
Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Zhongshan Road 457, Dalian 116023, China.
Molecules. 2023 Sep 23;28(19):6773. doi: 10.3390/molecules28196773.
In the healing of wounds, human-like collagen (hCol) is essential. However, collagen-based composite dressings have poor stability in vivo, which severely limits their current therapeutic potential. Based on the above, we have developed a recombinant fusion protein named hCol-ELP, which consists of hCol and an elastin-like peptide (ELP). Then, we examined the physicochemical and biological properties of hCol-ELP. The results indicated that the stability of the hCol-ELP fusion protein exhibited a more compact and homogeneous lamellar microstructure along with collagen properties, it was found to be significantly superior to the stability of free hCol. The compound hCol-ELP demonstrated a remarkable capacity to induce the proliferation and migration of mouse embryo fibroblast cells (NIH/3T3), as well as enhance collagen synthesis in human skin fibroblasts (HSF) when tested in vitro. In vivo, hCol-ELP demonstrated significant enhancements in healing rate and a reduction in the time required for scab removal, thereby exhibiting a scar-free healing effect. The findings provide a crucial theoretical foundation for the implementation of an hCol-ELP protein dressing in fields associated with the healing of traumatic injuries.
在创伤愈合过程中，类人胶原蛋白（hCol）是必不可少的。然而，基于胶原蛋白的复合敷料在体内的稳定性较差，这严重限制了其目前的治疗潜力。基于这一点，我们开发了一种名为 hCol-ELP 的重组融合蛋白，它由 hCol 和弹性蛋白样肽（ELP）组成。然后，我们研究了 hCol-ELP 的物理化学和生物学特性。结果表明，hCol-ELP 融合蛋白的稳定性表现出更紧凑和均匀的层状微结构以及胶原蛋白特性，其稳定性明显优于游离 hCol。在体外实验中，hCol-ELP 被发现能够显著促进小鼠胚胎成纤维细胞（NIH/3T3）的增殖和迁移，并增强人皮肤成纤维细胞（HSF）的胶原蛋白合成。在体内，hCol-ELP 显著提高了愈合速度，减少了结痂去除所需的时间，从而实现了无疤痕的愈合效果。这些发现为 hCol-ELP 蛋白敷料在创伤愈合等相关领域的应用提供了重要的理论基础。
