Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand.
Institute of Pharmacy/Pharmacognosy, CCB-Center for Chemistry and Biomedicine, University of Innsbruck, 6020 Innsbruck, Austria.
Molecules. 2023 Sep 28;28(19):6837. doi: 10.3390/molecules28196837.
Flavones are major compounds found in several parts of (). The quantification of multiple components by one marker (QAMS) method and the high-performance liquid chromatography (HPLC) method were developed for the quantitative analysis of extracts from the young fruits, green mature fruits, dry pod coats and seeds of . Oroxin A, oroxin B and chrysin-7--glucuronide were identified in the extracts. Oroxylin A and 5-hydroxymethylfurfural were isolated and structurally identified from the pod coat and young fruit extracts, respectively. From the HPLC analysis of the seven major flavones in the extracts, baicalin was the major compound in all extracts investigated (0.4-11% / of the extract). All flavone contents were low in the young fruit extract (<1% / of the extract). The green mature fruit and dry pod coat extracts showed similar constituent compositions. They contained small amounts of baicalin and oroxylin A, which were found only in these two extracts. Oroxylin A could be used as a marker to indicate the maturity of fruits, while 5-hydroxymethylfurfural could be used as a marker for the young fruits. Baicalin was found to be a suitable single marker to calculate the contents of all flavones in the extracts.
类黄酮是（）几个部位的主要化合物。建立了一种多组分单标记（QAMS）方法和高效液相色谱（HPLC）方法，用于定量分析的提取物从年轻的水果、绿色成熟的水果、干豆荚和种子。在提取物中鉴定出奥罗西林 A、奥罗西林 B 和白杨素-7-O-葡萄糖醛酸苷。从豆荚和幼果提取物中分离并结构鉴定出奥罗西林 A 和 5-羟甲基糠醛。从提取物中七种主要类黄酮的 HPLC 分析可知，黄芩素是所有提取物中含量最高的化合物（占提取物的 0.4-11%）。在幼果提取物中，所有类黄酮含量均较低（占提取物的<1%）。绿色成熟果实和干豆荚提取物具有相似的组成成分。它们只含有少量的黄芩素和奥罗西林 A，这两种提取物中都有。奥罗西林 A 可作为指示果实成熟度的标记物，而 5-羟甲基糠醛可作为幼果的标记物。黄芩素可作为计算提取物中所有类黄酮含量的合适单标记物。
