Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, China; College of Environment and Resources, Guangxi Normal University, Guilin 541004, China.
College of Environment and Resources, Guangxi Normal University, Guilin 541004, China.
J Hazard Mater. 2024 Jan 15;462:132761. doi: 10.1016/j.jhazmat.2023.132761. Epub 2023 Oct 11.
Bidens pilosa L. (B. pilosa) has great potential for the phytoremediation of cadmium (Cd)-contaminated soils. However, the molecular mechanism underlying Cd tolerance and detoxification in B. pilosa is still unclear. In the present study, a 4D label-free quantification technique combined with liquid chromatography-parallel reaction monitoring mass spectrometry was used to explore the stress response mechanism of B. pilosa. Proteomic analysis revealed 213 and 319 differentially expressed proteins (DEPs) in the roots and leaves of B. pilosa, respectively, and 12 target proteins were selected for further analysis. SWISS-MODEL was used to predict the 3D structures of the target proteins. The cation-ATPase-N structural domain and an ATPase-E1-E2 motif, which help to regulate ATPase function, were detected in the TR10519_c0_g1_ORF protein. In addition, the TR6620_c0_g1_ORF_1 and TR611_c1_g1_ORF proteins contained peroxidase-1 and peroxidase-2 motifs. The TR11239_c0_g1_ORF protein was found to belong to the Fe-SOD family, to have a dimeric structure and to contain a relatively high proportion of α-helices but few β-sheets, which play important roles in reactive oxygen intermediate scavenging. Thus, the current study provides an overview of the proteomic response of B. pilosa in scavenging of Cd-induced reactive oxygen intermediates and reveals key proteins involved in the stress response of B. pilosa under Cd exposure.
鬼针草（Bidens pilosa）具有很大的潜力可用于修复镉（Cd）污染土壤。然而，鬼针草耐受和解毒 Cd 的分子机制尚不清楚。本研究采用 4D 无标记定量技术结合液相色谱-平行反应监测质谱法，探讨了鬼针草的胁迫响应机制。蛋白质组学分析分别在鬼针草的根和叶中发现了 213 种和 319 种差异表达蛋白（DEPs），并选择了 12 种靶蛋白进行进一步分析。SWISS-MODEL 用于预测靶蛋白的 3D 结构。在 TR10519_c0_g1_ORF 蛋白中检测到阳离子-ATPase-N 结构域和有助于调节 ATPase 功能的 ATPase-E1-E2 基序。此外，TR6620_c0_g1_ORF_1 和 TR611_c1_g1_ORF 蛋白含有过氧化物酶-1 和过氧化物酶-2 基序。TR11239_c0_g1_ORF 蛋白属于 Fe-SOD 家族，具有二聚体结构，含有较高比例的α-螺旋和较少的β-折叠，在清除活性氧中间产物方面发挥重要作用。因此，本研究概述了鬼针草清除 Cd 诱导的活性氧中间产物的蛋白质组响应，并揭示了 Cd 暴露下鬼针草胁迫响应中的关键蛋白。
