Zheng Xiaofeng, Wang Xin, Li Pei, Zhou Yu, Zhu Xihui, Hu Zimeng, Wang Hui, Chen Mianmian, Huo Xiang, Liu Yingyu, Zhang Wei
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, 830052, China.
BMC Microbiol. 2025 Mar 26;25(1):169. doi: 10.1186/s12866-025-03895-8.
We engineered novel T5-like bacteriophage (phage) with extended host ranges by editing the long-tail fibers (PB3 and PB4) to combat Salmonella Enteritidis. By replacing the long-tail fibers PB3 and PB4 regions of phage PH204 with those from phage SP76, we created phages RPA and RPB, which exhibited expanded host ranges, lysing 54 strains compared to the original 30 strains. These phages retained the biological characteristics of PH204, including temperature, pH stability and adsorption rate. In milk, RPA and RPB inhibited Salmonella ZWSA605 growth, reducing bacterial counts to 1.51 log10 CFU/mL and 2.18 log10 CFU/mL after 8 h, respectively. Although the bacteriolytic activity of recombinant phages is lower than that of the parent phage, our findings suggest that these phages hold promise as candidates for future phage biocontrol applications in food.
我们通过编辑长尾纤维（PB3和PB4）来对抗肠炎沙门氏菌，构建了具有扩展宿主范围的新型T5样噬菌体。通过用噬菌体SP76的长尾纤维替换噬菌体PH204的长尾纤维PB3和PB4区域，我们创建了噬菌体RPA和RPB，它们表现出扩大的宿主范围，与原来的30株相比，能裂解54株菌株。这些噬菌体保留了PH204的生物学特性，包括温度、pH稳定性和吸附率。在牛奶中，RPA和RPB抑制了肠炎沙门氏菌ZWSA605的生长，8小时后细菌计数分别降至1.51 log10 CFU/mL和2.18 log10 CFU/mL。虽然重组噬菌体的溶菌活性低于亲本噬菌体，但我们的研究结果表明，这些噬菌体有望成为未来食品中噬菌体生物防治应用的候选者。
