Department of Biomedical Engineering, Middle East Technical University, Ankara, 06800, Turkey.
Department of Biomedical Engineering, Middle East Technical University, Ankara, 06800, Turkey; Department of Engineering Sciences, Middle East Technical University, Ankara, 06800, Turkey.
J Mech Behav Biomed Mater. 2023 Dec;148:106185. doi: 10.1016/j.jmbbm.2023.106185. Epub 2023 Oct 10.
Bioceramic/polymer composites have dragged a lot of attention for treating hard tissue damage in recent years. In this study, we synthesized barium-doped baghdadite (Ba-BAG), as a novel bioceramic, and later developed fibrous composite poly (hydroxybutyrate) co (hydroxyvalerate)- polycaprolactone (PHBV-PCL) scaffolds containing different amounts of baghdadite (BAG) and Ba-BAG, intended to be used in bone regeneration. Our results demonstrated that BAG and Ba-doped BAG powders were synthesized successfully using the sol-gel method and their microstructural, physicochemical, and cytotoxical properties results were evaluated. In the following, PHBV/PCL composite scaffolds containing different amounts of BAG and Ba-BAG (1, 3, and 5 wt%) were produced by the wet electrospinning method. The porosity of scaffolds decreased from 78% to 72% in Ba-BAG-incorporated PHBV/PCL scaffolds. The compressive strength of the scaffolds was between 4.69 and 9.28 kPa, which was increased to their maximum values in the scaffolds with Ba-BAG. The presence of BAG and Ba-BAG in the polymer scaffolds resulted in increasing bioactivity, and it was introduced as a suitable way to control the degradation rate of scaffolds. The presence of the BAG component was a major reason for higher cell proliferation in reinforced PHBV/PCL polymeric scaffolds, while Ba existence played its influential role in the higher osteogenic activity of cells on Ba-BAG incorporated PHBV/PCL scaffolds. Thus, the incorporation of Ba-BAG bioceramic materials into the structure of polymeric PHBV/PCL scaffolds promoted their various properties, and allow these scaffolds to be used as promising candidates in bone tissue engineering applications.
近年来，生物陶瓷/聚合物复合材料在治疗硬组织损伤方面引起了广泛关注。在本研究中，我们合成了钡掺杂钡铝榴石（Ba-BAG）作为一种新型生物陶瓷，随后开发了含有不同量钡铝榴石（BAG）和 Ba-BAG 的纤维状复合聚（羟基丁酸酯）共（羟基戊酸酯）-聚己内酯（PHBV-PCL）支架，旨在用于骨再生。我们的结果表明，成功地使用溶胶-凝胶法合成了 BAG 和 Ba 掺杂的 BAG 粉末，并对其微观结构、物理化学和细胞毒性特性进行了评估。接下来，通过湿法静电纺丝法制备了含有不同量 BAG 和 Ba-BAG（1、3 和 5wt%）的 PHBV/PCL 复合支架。支架的孔隙率从 BAG 掺入的 PHBV/PCL 支架中的 78%降低到 72%。支架的压缩强度在 4.69 和 9.28kPa 之间，在含有 Ba-BAG 的支架中达到最大值。BAG 和 Ba-BAG 存在于聚合物支架中导致生物活性增加，并被引入作为控制支架降解率的合适方法。BAG 成分的存在是增强 PHBV/PCL 聚合物支架中细胞增殖的主要原因，而 Ba 的存在对 Ba-BAG 掺入的 PHBV/PCL 支架中细胞的更高成骨活性发挥了影响作用。因此，将 Ba-BAG 生物陶瓷材料掺入聚合物 PHBV/PCL 支架的结构中可改善其各种性能，并使这些支架成为骨组织工程应用中的有前途的候选材料。
