From the Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran.
Department of Immunogenetics, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
ASAIO J. 2020 Aug;66(8):966-973. doi: 10.1097/MAT.0000000000001094.
Today, composite scaffolds fabricated by natural and synthetic polymers have attracted a lot of attention among researchers in the field of tissue engineering, and given their combined properties that can play a very useful role in repairing damaged tissues. In the current study, aloe vera-derived gel-blended poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanofibrous scaffold was fabricated by electrospinning, and then, PHBV and PHBV gel fabricated scaffolds characterized by scanning electron microscope, protein adsorption, cell attachment, tensile and cell's viability tests. After that, osteogenic supportive property of the scaffolds was studied by culturing of human-induced pluripotent stem cells on the scaffolds under osteogenic medium and evaluating of the common bone-related markers. The results showed that biocompatibility of the PHBV nanofibrous scaffold significantly improved when combined with the aloe vera gel. In addition, higher amounts of alkaline phosphatase activity, mineralization, and bone-related gene and protein expression were detected in stem cells when grown on PHBV-gel scaffold in comparison with those stem cells grown on the PHBV and culture plate. Taken together, it can be concluded that aloe vera gel-blended PHBV scaffold has a great promising osteoinductive potential that can be used as a suitable bioimplant for bone tissue engineering applications to accelerate bone regeneration and also degraded completely along with tissue regeneration.
今天，由天然和合成聚合物制成的复合支架在组织工程领域的研究人员中引起了极大的关注，并且由于其组合性能，它们在修复受损组织方面可以发挥非常有用的作用。在本研究中，通过静电纺丝制备了由芦荟衍生的凝胶共混聚（3-羟基丁酸酯-co-3-羟基戊酸酯）（PHBV）纳米纤维支架，然后通过扫描电子显微镜、蛋白质吸附、细胞附着、拉伸和细胞活力测试对 PHBV 和 PHBV 凝胶制备的支架进行了表征。之后，通过在成骨培养基中培养人诱导多能干细胞并评估常见的骨相关标志物来研究支架的成骨支持特性。结果表明，当与芦荟凝胶结合时，PHBV 纳米纤维支架的生物相容性显著提高。此外，与在 PHBV 支架和培养板上生长的干细胞相比，在 PHBV-凝胶支架上生长的干细胞中碱性磷酸酶活性、矿化以及骨相关基因和蛋白表达的量更高。综上所述，可以得出结论，芦荟凝胶共混 PHBV 支架具有很大的成骨诱导潜力，可作为骨组织工程应用的合适生物植入物，以加速骨再生，并随着组织再生完全降解。
