Giwa Abdulmoseen Segun, Ali Nasir, Akhter Mohammed Salim
School of Environment and Civil Engineering, Nanchang Institute of Science and Technology, Nanchang, 330108, China.
Institute of Biotechnology Genetic Engineering, The University of Agriculture, Peshawar, 25130, Khyber Pakhtunkhwa, Pakistan.
Mol Biotechnol. 2023 Oct 15. doi: 10.1007/s12033-023-00900-1.
The economic exploration of renewable energy resources has hot fundamentals among the countries besides dwindling energy resources and increasing public pressure. Cellulose accumulation is a major bio-natural resource from agricultural waste. Cellulases are the most potential enzymes that systematically degrade cellulosic biomass into monomers which could be further processed into several efficient value-added products via chemical and biological reactions including useful biomaterial for human benefits. This could lower the environmental risks problems followed by an energy crisis. Cellulases are mainly synthesized by special fungal genotypes. The strain Trichoderma orientalis could highly express cellulases and was regarded as an ideal strain for further research, as the genetic tools have found compatibility for cellulose breakdown by producing effective cellulose-degrading enzymes. This strain has found a cellulase production of about 35 g/L that needs further studies for advancement. The enzyme activity of strain Trichoderma orientalis needed to be further improved from a molecular level which is one of the important methods. Considering synthetic biological approaches to unveil the genetic tools will boost the knowledge about commercial cellulases bioproduction. Several genetic transformation methods were significantly cited in this study. The transformation approaches that are currently researchers are exploring is transcription regulatory factors that are deeply explained in this study, that are considered essential regulators of gene expression.
除了能源资源日益减少和公众压力不断增加外，可再生能源资源的经济开发在各国也有热门的基础。纤维素积累是农业废弃物中的一种主要生物自然资源。纤维素酶是最具潜力的酶，能将纤维素生物质系统地降解为单体，这些单体可通过化学和生物反应进一步加工成多种高效增值产品，包括对人类有益的生物材料。这可以降低能源危机带来的环境风险问题。纤维素酶主要由特殊的真菌基因型合成。东方木霉菌株能高效表达纤维素酶，由于遗传工具已发现其通过产生有效的纤维素降解酶来分解纤维素具有兼容性，因此被视为进一步研究的理想菌株。该菌株的纤维素酶产量约为35克/升，需要进一步研究以取得进展。从分子水平进一步提高东方木霉菌株的酶活性是重要方法之一。考虑到合成生物学方法来揭示遗传工具将增进对商业纤维素酶生物生产的了解。本研究显著引用了几种遗传转化方法。目前研究人员正在探索的转化方法是转录调节因子，本研究对此进行了深入解释，转录调节因子被认为是基因表达的关键调节因子。
