School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China; School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China.
School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China.
Ultrason Sonochem. 2023 Nov;100:106635. doi: 10.1016/j.ultsonch.2023.106635. Epub 2023 Oct 7.
The study aimed to improve the extraction rate of Platycodon grandiflorum roots polysaccharides (PGPs) using ultrasound-assisted extraction (UAE). A comparative analysis was undertaken to evaluate polysaccharides content, molecular weight distribution, monosaccharide composition, preliminary structure, antioxidant, and hypoglycemic activity of UAE in comparison with heating water extraction (HWE). The optimum extraction conditions included a liquid-to-material ratio of 20 mL/g, ultrasonic power of 150 W, extraction temperature of 70 ℃, and extraction time of 20 min, resulting in a significantly greater polysaccharides (12.011 ± 0.91 %) compared to HWE (7.62 ± 0.18 %). Through Sephacryl S-100 column elution, two homogenous fraction (PGP-U extracted with UAE and PGP-H extracted with HAE) were obtained. The molecular weight of PGP-U and PGP-H was 3.14 kDa and 3.44 kDa, respectively, mainly composed of different proportions of fourteen monosaccharides. Fourier transform infrared spectroscopy (FT-IR) and Nuclear Magnetic Resonance (NMR) spectra experiment results showed that the two polysaccharides were pyranose ring with α- and β-glycoside bond. PGP-U and PGP-H exhibited specific antioxidant activities, encompassing total reducing force, scavenging of DPPH radicals, ABTs radicals and hydroxyl radicals in vitro, along with mitigation of HO-induced damage in HepG2 cells. Moreover, PGP-U exerted significantly stronger inhibitory activities against α-amylase and α-glucosidase and could significantly enhances the glucose uptake capacity and intracellular glycogen content of insulin-resistant HepG2 (IR-HepG2) cells.
本研究旨在利用超声辅助提取（UAE）提高桔梗根多糖（PGPs）的提取率。通过比较分析，评估了 UAE 与加热水提取（HWE）相比，多糖含量、分子量分布、单糖组成、初步结构、抗氧化和降血糖活性。最佳提取条件为液料比 20 mL/g、超声功率 150 W、提取温度 70℃、提取时间 20 min，与 HWE（7.62±0.18%）相比，多糖得率显著提高（12.011±0.91%）。通过 Sephacryl S-100 柱洗脱，得到两种均一的组分（分别用 UAE 提取的 PGP-U 和用 HAE 提取的 PGP-H）。PGP-U 和 PGP-H 的分子量分别为 3.14 kDa 和 3.44 kDa，主要由十四种单糖以不同比例组成。傅里叶变换红外光谱（FT-IR）和核磁共振（NMR）光谱实验结果表明，两种多糖均为吡喃糖环，具有α-和β-糖苷键。PGP-U 和 PGP-H 表现出特定的抗氧化活性，包括总还原力、体外清除 DPPH 自由基、ABTs 自由基和羟自由基，以及减轻 HO 诱导的 HepG2 细胞损伤。此外，PGP-U 对α-淀粉酶和α-葡萄糖苷酶表现出更强的抑制活性，能够显著增强胰岛素抵抗 HepG2（IR-HepG2）细胞的葡萄糖摄取能力和细胞内糖原含量。
