Huang Yuanshe, Zhang Lai, Liu Tianlei, Liang E
Agriculture College, Innovation Center for Efficient Agricultural of Guizhou Mountain Characteristics, Anshun University, Anshun, Guizhou 561000, P.R. China.
Exp Ther Med. 2023 Sep 20;26(5):513. doi: 10.3892/etm.2023.12212. eCollection 2023 Nov.
Forkhead box D1 (FOXD1) expression is upregulated in various types of human cancer. To the best of our knowledge, the roles of FOXD1 in prostate cancer (PC) remain largely unknown. The Cancer Genome Atlas dataset was used for the bioinformatics analysis of FOXD1 in PC. FOXD1 expression levels in normal immortalized human prostate epithelial cells (RWPE-1) and prostate cancer cells were detected by reverse transcription-quantitative PCR. PC cell viability was detected using Cell Counting Kit-8 assay. Transwell assays were performed to assess the migration and invasion of PC cells. Luciferase reporter gene assay was used to validate the association between FOXD1 and lamin (LMN)B1. LMNB1 is an important part of the cytoskeleton, which serves an important role in the process of tumor occurrence and development, regulating apoptosis and DNA repair. FOXD1 expression was upregulated in PC tissues, with its high expression being associated with clinical stage and survival in PC. Knockdown of FOXD1 inhibited viability, migration and invasion of PC cells. FOXD1 positively regulated LMNB1 expression. The effect of FOXD1 knockdown on PC cells was reversed by LMNB1 overexpression. In conclusion, FOXD1, positively regulated by LMNB1, served as an oncogene in PC and may be a potential biomarker and treatment target for PC.
叉头框D1（FOXD1）在多种人类癌症中表达上调。据我们所知，FOXD1在前列腺癌（PC）中的作用仍 largely unknown。利用癌症基因组图谱数据集对PC中的FOXD1进行生物信息学分析。通过逆转录定量PCR检测正常永生化人前列腺上皮细胞（RWPE-1）和前列腺癌细胞中FOXD1的表达水平。使用细胞计数试剂盒-8检测法检测PC细胞活力。进行Transwell检测以评估PC细胞的迁移和侵袭能力。荧光素酶报告基因检测用于验证FOXD1与核纤层蛋白（LMN）B1之间的关联。LMNB1是细胞骨架的重要组成部分，在肿瘤发生和发展过程中起重要作用，调节细胞凋亡和DNA修复。FOXD1在PC组织中表达上调，其高表达与PC的临床分期和生存相关。敲低FOXD1可抑制PC细胞的活力、迁移和侵袭。FOXD1正向调节LMNB1的表达。LMNB1过表达可逆转FOXD1敲低对PC细胞的影响。总之，受LMNB1正向调节的FOXD1在PC中作为一种癌基因发挥作用，可能是PC的潜在生物标志物和治疗靶点。
