Cao Yu, Wang Longfei, Zhang Xiumei, Lu Yi, Wei Yan, Liang Ziwei, Hu Yinchun, Huang Di
Department of Biomedical Engineering, Research Center for Nano-Biomaterials & Regenerative Medicine, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
Shanxi-Zheda Institute of Advanced Materials and Chemical Engineering, Taiyuan 030032, China.
Regen Biomater. 2023 Sep 9;10:rbad081. doi: 10.1093/rb/rbad081. eCollection 2023.
Rapid post-wound closure is necessary to avoid wound infection and promote scar-free healing when skin trauma occurs. In this study, new types of hydrogel dressings with adjustable contractility were fabricated based on -isopropyl acrylamide/sodium alginate/graphene oxide (P/SA/GO). Then, the chitosan (CS) solution was used as a bridging polymer to achieve tissue adhesion to the hydrogel. The results show that the hydrogel based on poly(-isopropyl acrylamide) (PNIPAM) not only has the ability to self-shrink but also can adjust the rate of shrinkage through near-infrared thermal stimulation. At the same time, high adhesion strength (7.86 ± 1.22 kPa) between the tissue and the dressing is achieved through the introduction of bridging polymers (CS), and the coating area of the bridging polymer can be adjusted to achieve regional adhesion. The mouse total skin defects experiments have shown that sutures-free wound closure in the early stages of wound healing could be obtained by adjusting the material temperature. Besides, the dressings can promote scar-free wound healing by reducing inflammatory cell infiltration and collagen deposition. These results indicate that double-crosslinked PNIPAM-based hydrogel dressings with adjustable adhesion and contractility proposed in this study provide a candidate material for achieving trackless wound healing.
皮肤创伤发生时，快速闭合伤口对于避免伤口感染和促进无瘢痕愈合至关重要。在本研究中，基于聚异丙基丙烯酰胺/海藻酸钠/氧化石墨烯（P/SA/GO）制备了具有可调收缩性的新型水凝胶敷料。然后，使用壳聚糖（CS）溶液作为桥联聚合物，使水凝胶实现与组织的黏附。结果表明，基于聚（异丙基丙烯酰胺）（PNIPAM）的水凝胶不仅具有自收缩能力，还能通过近红外热刺激调节收缩速率。同时，通过引入桥联聚合物（CS），实现了组织与敷料之间的高黏附强度（7.86±1.22kPa），并且可以调节桥联聚合物的涂层面积以实现区域黏附。小鼠全层皮肤缺损实验表明，通过调节材料温度，在伤口愈合早期可实现免缝合伤口闭合。此外，该敷料可通过减少炎性细胞浸润和胶原蛋白沉积来促进无瘢痕伤口愈合。这些结果表明，本研究中提出的具有可调黏附性和收缩性的双交联PNIPAM基水凝胶敷料为实现无痕伤口愈合提供了一种候选材料。
