Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong, PR China.
Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong, PR China; Department of Orthopedics, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, PR China; Department of Emergency surgery, Shengli Clinical Medical College of Fujian Medical University, Fujian Provincial Hospital, Fuzhou, PR China.
Biomater Adv. 2022 Mar;134:112560. doi: 10.1016/j.msec.2021.112560. Epub 2021 Nov 24.
In addition to preventing infection and promoting angiogenesis, novel hydrogel dressings are highly expected to possess the potential to scavenge reactive oxygen species (ROS) and reduce inflammatory responses during the wound healing process. In this study, we designed and fabricated a hydrogel dressing (CBD/Alg@Zn) containing cannabidiol (CBD) based on the ion crosslinked interaction between Zn ions and the alginate polymer (Alg). The as-fabricated hydrogel exhibited a suitable swelling ratio, sufficient thermal stability, and stable rheological property. In vitro biological activity experiments indicated that the hydrogel has good biocompatibility, antibacterial activity, and angiogenesis properties. Moreover, it could significantly scavenge DPPH (2,2-diphenyl-1-picrylhydrazyl) free radicals and reduce the inflammatory response. In vivo studies revealed that the CBD/Alg@Zn hydrogel significantly facilitated the wound healing process by controlling the inflammatory infiltration, promoting collagen deposition and the granulation tissue, and benefiting the formation of blood vessels. We, therefore, suggested that CBD/Alg@Zn hydrogel should be a potential candidate material for wound dressing and skin tissue engineering.
除了预防感染和促进血管生成外，新型水凝胶敷料还高度期望具有在伤口愈合过程中清除活性氧 (ROS) 和减少炎症反应的潜力。在本研究中，我们设计并制备了一种含有大麻二酚 (CBD) 的水凝胶敷料 (CBD/Alg@Zn)，该水凝胶基于 Zn 离子与藻酸盐聚合物 (Alg) 之间的离子交联相互作用。所制备的水凝胶具有合适的溶胀比、足够的热稳定性和稳定的流变性能。体外生物活性实验表明，水凝胶具有良好的生物相容性、抗菌活性和血管生成特性。此外，它还可以显著清除 DPPH（2,2-二苯基-1-苦基肼）自由基并减轻炎症反应。体内研究表明，CBD/Alg@Zn 水凝胶通过控制炎症浸润、促进胶原蛋白沉积和肉芽组织形成以及有利于血管形成，显著促进了伤口愈合过程。因此，我们认为 CBD/Alg@Zn 水凝胶应该是伤口敷料和皮肤组织工程的潜在候选材料。
