Research Group on Bioengineering, Membrane Technology and Energetics, Faculty of Engineering, University of Pannonia, Egyetem str. 10, Veszprém, 8200, Hungary.
World J Microbiol Biotechnol. 2023 Oct 16;39(12):346. doi: 10.1007/s11274-023-03797-9.
Itaconic acid is an important bio-based chemical. The present study aims to evaluate the applicability of semi-continuous fermentation technique for itaconic acid production by Aspergillus terreus. The fermentation is planned to be connected with bipolar membrane electrodialysis unit for acid recovery. This process allows the reuse of residual glucose from the effluent. Our particular attention was focused on the effect of glucose concentration. Two different glucose supplementation strategies were tested: constant glucose concentration in the refilling medium and adjusted glucose concentration in order to maintain a continuously high - 120 g/L - glucose concentration in the fermentor. The itaconic acid titre, yield and productivity for the 24 h time periods between draining/refilling interventions were investigated. The constantly high glucose concentration in the fermentor resulted in doubled biomass formation. The average itaconic acid titre was 32.9 ± 2.7 g/L. The producing strain formed numerous spores during semi-continuous fermentation that germinated continuously. Yield and volumetric productivity showed a periodic pattern during the procedure.
衣康酸是一种重要的生物基化学品。本研究旨在评估半连续发酵技术在土曲霉生产衣康酸中的适用性。发酵计划与双极膜电渗析装置连接，以回收酸。该过程允许再利用废水中的残留葡萄糖。我们特别关注葡萄糖浓度的影响。测试了两种不同的葡萄糖补充策略：补料培养基中葡萄糖浓度恒定和葡萄糖浓度调整，以保持发酵罐中持续高浓度-120 g/L-葡萄糖。研究了排液/补料干预之间 24 小时时间段的衣康酸产量、产率和生产力。发酵罐中持续高浓度的葡萄糖导致生物量形成增加一倍。平均衣康酸产量为 32.9±2.7 g/L。在半连续发酵过程中，生产菌株形成了大量的孢子，这些孢子不断发芽。在该过程中，产率和容积生产力呈现周期性模式。
