Department of Analytical Chemistry, Institute of Chemistry, Federal University of Bahia, Salvador, BA, Brazil.
Adv Exp Med Biol. 2023;1439:149-183. doi: 10.1007/978-3-031-41741-2_7.
Microbial metabolomics has gained significant interest as it reflects the physiological state of microorganisms. Due to the great variability of biological organisms, in terms of physicochemical characteristics and variable range of concentration of metabolites, the choice of sample preparation methods is a crucial step in the metabolomics workflow and will reflect on the quality and reliability of the results generated. The procedures applied to the preparation of microbial samples will vary according to the type of microorganism studied, the metabolomics approach (untargeted or targeted), and the analytical platform of choice. This chapter aims to provide an overview of the sample preparation workflow for microbial metabolomics, highlighting the pre-analytical factors associated with cultivation, harvesting, metabolic quenching, and extraction. Discussions focus on obtaining intracellular and extracellular metabolites. Finally, we introduced advanced sample preparation methods based on automated systems.
微生物代谢组学作为反映微生物生理状态的方法，受到了广泛关注。由于生物组织在理化特性和代谢物浓度变化范围方面存在很大的可变性，因此选择合适的样品制备方法是代谢组学工作流程中的关键步骤，这将直接影响到所生成结果的质量和可靠性。应用于微生物样品制备的程序将根据所研究的微生物类型、代谢组学方法（靶向或非靶向）以及所选的分析平台而有所不同。本章旨在概述微生物代谢组学的样品制备工作流程，重点介绍与培养、收获、代谢物猝灭和提取相关的分析前因素。讨论集中在获得细胞内和细胞外代谢物上。最后，我们介绍了基于自动化系统的先进样品制备方法。
