Gustafsson J E
Clin Chem. 1979 Aug;25(8):1476-9.
A homogeneous enzyme immunoassay (EMIT) for serum thyroxine determination has been evaluated. It was also compared to our routine method, a radioimmunoassay with use of a commercial kit, Tetra-Tab RIA. Within-day precision was very similar for the two methods, the CV ranging from about 2.5% at 150 nmol of thyroxine per liter to about 5.7% at 25 nmol/L. The two methods were compared by running 100 patients' serum samples. The resulting linear regression equation was: y = 1.088x - 17.9, with a correlation coefficient r = 0.979. The deviation from the theoretical line, y = x, wash shown to be primarily caused by the calibration of the methods, shown by running the EMIT calibrators in the radioimmunoassay method and vice versa. The choice of thyroxine method in the individual laboratory will then depend on considerations other than methodological factors.
一种用于血清甲状腺素测定的均相酶免疫分析（EMIT）方法已得到评估。它还与我们的常规方法，即使用商业试剂盒Tetra-Tab RIA的放射免疫分析进行了比较。两种方法的日内精密度非常相似，变异系数（CV）范围从每升150纳摩尔甲状腺素时的约2.5%到25纳摩尔/升时的约5.7%。通过检测100例患者的血清样本对两种方法进行了比较。所得线性回归方程为：y = 1.088x - 17.9，相关系数r = 0.979。与理论线y = x的偏差主要是由方法的校准引起的，这通过在放射免疫分析方法中检测EMIT校准品以及反之在EMIT方法中检测放射免疫分析校准品得以体现。那么，各个实验室对甲状腺素测定方法的选择将取决于方法学因素以外的其他考虑因素。
