Zoology Department, Faculty of Science (Boys), Al-Azhar University, Cairo, 11884, Egypt.
Biological Prevention Department, Ministry of Defense, Cairo, 11766, Egypt.
Histochem Cell Biol. 2024 Feb;161(2):165-181. doi: 10.1007/s00418-023-02244-y. Epub 2023 Oct 17.
Burn injuries pose a significant healthcare burden worldwide, often leading to long-term disabilities and reduced quality of life. To explore the impacts of the transplantation of mesenchymal stem cells (MSCs) on the healing of burns and the levels of serum cytokines, 60 fully grown Sprague-Dawley rats were randomly divided into three groups (n = 20 each): group I (control), group II (burn induction), and group III (burn induction + bone marrow (BM)-MSC transplantation). Groups II and III were further divided into four subgroups (n = 5 each) based on euthanasia duration (7, 14, 21, and 28 days post transplant). The experiment concluded with an anesthesia overdose for rat death. After 7, 14, 21, and 28 days, the rats were assessed by clinical, laboratory, and histopathology investigations. The results revealed significant improvements in burn healing potentiality in the group treated with MSC. Furthermore, cytokine levels were measured, with significant increases in interleukin (IL)-6 and interferon alpha (IFN) observed, while IL-10 and transforming growth factor beta (TGF-β) decreased at 7 days and increased until 28 days post burn. Also, the group that underwent the experiment exhibited increased levels of pro-inflammatory cytokines and the anti-inflammatory cytokine IL-10 when compared to the control group. Histological assessments showed better re-epithelialization, neovascularization, and collagen deposition in the experimental group, suggesting that MSC transplantation in burn wounds may promote burn healing by modulating the immune response and promoting tissue regeneration.
烧伤对全球的医疗保健造成了重大负担，常导致长期残疾和生活质量下降。为了探究间充质干细胞（MSCs）移植对烧伤愈合和血清细胞因子水平的影响，将 60 只成年 Sprague-Dawley 大鼠随机分为三组（每组 n = 20）：I 组（对照组）、II 组（烧伤诱导）和 III 组（烧伤诱导+骨髓（BM）-MSC 移植）。根据安乐死时间（移植后 7、14、21 和 28 天），II 组和 III 组进一步分为四个亚组（每组 n = 5）。实验以大鼠麻醉过量死亡结束。在 7、14、21 和 28 天后，通过临床、实验室和组织病理学检查对大鼠进行评估。结果显示，MSC 治疗组的烧伤愈合潜能有显著改善。此外，还测量了细胞因子水平，发现白细胞介素（IL）-6 和干扰素 alpha（IFN）显著增加，而 IL-10 和转化生长因子 beta（TGF-β）在烧伤后 7 天减少，直到 28 天增加。此外，与对照组相比，实验组表现出更高水平的促炎细胞因子和抗炎细胞因子 IL-10。组织学评估显示，实验组的再上皮化、新生血管形成和胶原沉积更好，表明 MSC 移植可能通过调节免疫反应和促进组织再生来促进烧伤愈合。
