Simon Fraser University, Department of Biological Sciences, C2D2 Research Group, Burnaby, British Columbia, Canada.
Universidad Icesi, Natural Science Faculty, Department of Biology, Cali, Colombia.
PLoS Negl Trop Dis. 2023 Oct 17;17(10):e0011676. doi: 10.1371/journal.pntd.0011676. eCollection 2023 Oct.
The mosquito Aedes aegypti is the primary vector for all four serotypes of dengue viruses (DENV1-4), which infect millions across the globe each year. Traditional insecticide programs have been transiently effective at minimizing cases; however, insecticide resistance and habitat expansion have caused cases of DENV to surge over the last decade. There is an urgent need to develop novel vector control measures, but these are contingent on a detailed understanding of host-parasite interactions. Here, we have utilized lipidomics to survey the profiles of naturally DENV-resistant (Cali-MIB) or susceptible (Cali-S) populations of Ae. aegypti, isolated from Cali, Colombia, when fed on blood meals containing DENV. Control insects were fed on a DENV-free blood meal. Midguts were dissected from Cali-MIB and Cali-S females at three time points post-infectious blood meal, 18, 24 and 36h, to identify changes in the lipidome at key times associated with the entry, replication and exit of DENV from midgut cells. We used principal component analysis to visualize broad patterns in lipidomic profiles between the treatment groups, and significance analysis of microarray to determine lipids that were altered in response to viral challenge. These data can be used to identify molecules or metabolic pathways particular to the susceptible or refractory phenotypes, and possibly lead to the generation of stable, DENV-resistant strains of Ae. aegypti.
埃及伊蚊是所有四种登革热病毒（DENV1-4）的主要传播媒介，每年在全球范围内感染数百万人。传统的杀虫剂计划在减少病例方面曾短暂有效；然而，杀虫剂耐药性和栖息地扩张导致过去十年登革热病例激增。迫切需要开发新的病媒控制措施，但这取决于对宿主-寄生虫相互作用的详细了解。在这里，我们利用脂质组学来检测从哥伦比亚卡利分离的自然抗登革热（Cali-MIB）或易感（Cali-S）埃及伊蚊种群在摄入含有登革热病毒的血液时的脂质谱。对照昆虫以不含登革热病毒的血液为食。在感染传染性血液后 18、24 和 36 小时，从 Cali-MIB 和 Cali-S 雌性昆虫的中肠中分离出中肠，以确定与登革热病毒从中肠细胞进入、复制和退出相关的关键时间点脂质组的变化。我们使用主成分分析来可视化治疗组之间脂质组谱的广泛模式，并使用微阵列的显著性分析来确定对病毒挑战有反应的脂质。这些数据可用于鉴定与易感或耐药表型特别相关的分子或代谢途径，并可能导致产生稳定的抗登革热病毒的埃及伊蚊品系。
