Simon Fraser University, Department of Biological Sciences, C2D2 Research Group, Burnaby, BC, Canada.
Universidad Icesi, Natural Science Faculty, Centro Internacional de Entrenamiento e Investigaciones Médicas, Department of Biology, Vector Biology and Control, Cali, Colombia.
Mem Inst Oswaldo Cruz. 2021 May 28;116:e200547. doi: 10.1590/0074-02760200547. eCollection 2021.
Forty percent of the world's population live in areas where they are at risk from dengue fever, dengue hemorrhagic fever, and dengue shock syndrome. Dengue viruses are transmitted primarily by the mosquito Aedes aegypti. In Cali, Colombia, approximately 30% of field collected Ae. aegypti are naturally refractory to all four dengue serotypes.
Use RNA-sequencing to identify those genes that determine refractoriness in feral mosquitoes to dengue. This information can be used in gene editing strategies to reduce dengue transmission.
We employed a full factorial design, analyzing differential gene expression across time (24, 36 and 48 h post bloodmeal), feeding treatment (blood or blood + dengue-2) and strain (susceptible or refractory). Sequences were aligned to the reference Ae. aegypti genome for identification, assembled to visualize transcript structure, and analyzed for dynamic gene expression changes. A variety of clustering techniques was used to identify the differentially expressed genes.
We identified a subset of genes that likely assist dengue entry and replication in susceptible mosquitoes and contribute to vector competence.
The differential expression of specific genes by refractory and susceptible mosquitoes could determine the phenotype, and may be used to in gene editing strategies to reduce dengue transmission.
世界上有 40%的人口生活在可能感染登革热、登革出血热和登革休克综合征的地区。登革病毒主要通过埃及伊蚊传播。在哥伦比亚卡利，约 30%的野外采集的埃及伊蚊对所有四种登革热血清型天然具有抗性。
使用 RNA 测序来确定决定野栖蚊子对登革热抗性的那些基因。这些信息可用于基因编辑策略，以减少登革热的传播。
我们采用完全析因设计，分析了在血液摄入后 24、36 和 48 小时（24、36 和 48 小时）、喂养处理（血液或血液+登革热 2 型）和品系（敏感或抗性）时间点的差异基因表达。将序列与埃及伊蚊参考基因组进行比对以进行鉴定，组装以可视化转录本结构，并分析动态基因表达变化。使用了多种聚类技术来识别差异表达基因。
我们确定了一组可能有助于易感蚊子中登革热进入和复制的基因，并有助于媒介效能。
抗性和易感蚊子中特定基因的差异表达可能决定表型，并且可用于基因编辑策略以减少登革热的传播。
