Rajagopal Anjale, Varghese Jothi, Bhat Vinutha, Acharya Vibha, Kamath Vinayak
Department of Periodontology, Manipal College of Dental Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, India.
Department of Biochemistry, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, Karnataka, India.
Eur J Dent. 2024 May;18(2):526-533. doi: 10.1055/s-0043-1772567. Epub 2023 Oct 17.
Periodontal debridement involves conventional scaling and root planing (SRP) along with variant forms of adjunctive therapies. In the present clinical trial, we investigated if the adjunctive use of HybenX gel or diode laser along with SRP could provide a favorable outcome for the treatment of chronic periodontitis.
The present study involved 60 subjects diagnosed with chronic periodontitis who were randomly assigned as test groups (laser or HybenX) or control group (SRP alone). The primary outcomes of the clinical trial were pocket probing depth (PPD) and clinical attachment level (CAL), which was evaluated at baseline and at third-month time interval. Additionally, secondary outcomes included estimation of reduction in inflammatory mediators interleukin 1β (IL-1β) and tumor necrosis factor α (TNF-α) in gingival crevicular fluid using enzyme-linked immunosorbent assay at baseline and third-month intervals.
Normality determination was checked using Shapiro-Wilk test. Since the data was not normally distributed, nonparametric tests were applied. The comparison of clinical parameters between the groups was analyzed with Kruskal-Wallis test. Wilcoxon sign rank test was used to compare the pairwise comparison of clinical parameters among the groups from baseline to third-month follow-up. The inflammatory mediators at various time points were compared using a One-way analysis of variance test, and the inflammatory mediators in each study group were compared using a paired -test.
Both the test groups demonstrated a decrease in PPD and CAL. However, the HybenX group exhibited statistically significant reduction at the end of the third-month study interval compared to the laser group and SRP alone. Further, the secondary outcome IL-1β and TNF-α analysis exhibited statistically significant reduction in all the groups posttherapy.
The adjunctive application of HybenX gel yielded an advantage compared to laser and SRP for the treatment of chronic periodontitis.
Adjunctive use of the oral tissue desiccant (HybenX gel) combined with SRP improved the periodontal pocket disinfection process and enhanced tissue healing devoid of adverse effects.
牙周清创术包括传统的龈上洁治和根面平整（SRP）以及多种辅助治疗形式。在本临床试验中，我们研究了在SRP基础上联合使用海本X凝胶或二极管激光是否能为慢性牙周炎的治疗带来良好效果。
本研究纳入60例被诊断为慢性牙周炎的受试者，他们被随机分为试验组（激光组或海本X组）和对照组（仅SRP组）。临床试验的主要结局指标为牙周袋探诊深度（PPD）和临床附着水平（CAL），分别在基线和第三个月时进行评估。此外，次要结局指标包括在基线和第三个月时，通过酶联免疫吸附测定法评估龈沟液中炎症介质白细胞介素1β（IL-1β）和肿瘤坏死因子α（TNF-α）的减少情况。
使用夏皮罗-威尔克检验检查数据的正态性。由于数据不呈正态分布，因此应用非参数检验。使用Kruskal-Wallis检验分析组间临床参数的比较。使用Wilcoxon符号秩检验比较从基线到第三个月随访期间组间临床参数的两两比较。使用单向方差分析检验比较不同时间点的炎症介质，并使用配对t检验比较每个研究组中的炎症介质。
两个试验组的PPD和CAL均有所下降。然而，与激光组和仅SRP组相比，海本X组在第三个月研究期结束时显示出统计学上的显著降低。此外，次要结局指标IL-1β和TNF-α分析显示，所有组在治疗后均有统计学上的显著降低。
与激光和SRP相比，海本X凝胶的辅助应用在治疗慢性牙周炎方面具有优势。
口服组织干燥剂（海本X凝胶）与SRP联合使用可改善牙周袋消毒过程，并增强组织愈合，且无不良反应。
