Center for Pharmaceutical Biotechnology, School of Medicine and Pharmacy, Duy Tan University, Danang, 550000, Vietnam.
Sci Rep. 2023 Oct 17;13(1):17669. doi: 10.1038/s41598-023-43951-9.
Cultivation of Cordyceps militaris, a valuable medicinal and edible fungus, has dramatically increased in Vietnam since 2010. During industrial production, parasitic white molds were found to infect the mycelia and fruiting bodies of C. militaris causing significant quality and yield losses. Two different fungal strains were obtained from the mycelia and fruiting bodies of C. militaris in Danang mushroom farms and were characterized by morphological and multiple DNA markers analysis. The sequence alignment of ITS, LSU and rpb2 markers revealed that the pathogens are related to the type species Lecanicillium coprophilum and Calcarisporium cordycipiticola with more than 99% sequence identities. The growth characteristics and pathogenic activities of the two isolated species on their host C. militaris were also investigated. The phylogenetic analysis based on the ITS sequences showed that L. coprophilum WF2611 is closer to its host C. militaris than C. cordycipiticola NT1504. To our knowledge, this is the first worldwide report of C. militaris infected by L. coprophilum which would be an useful information on prevention and control of the disease and be helpful for the industrial cultivation of C. militaris.
自 2010 年以来，越南的药用和食用真菌——蛹虫草的种植规模急剧扩大。在工业生产过程中，发现寄生白霉菌感染了蛹虫草的菌丝和子实体，导致其质量和产量显著下降。在岘港蘑菇农场的蛹虫草菌丝体和子实体中分离到两种不同的真菌菌株，并通过形态学和多个 DNA 标记分析进行了鉴定。ITS、LSU 和 rpb2 标记的序列比对表明，这些病原菌与模式种粪生帚梗霉和虫草拟青霉关系密切，序列同一性超过 99%。还研究了两种分离种在其宿主蛹虫草上的生长特性和致病性。基于 ITS 序列的系统发育分析表明，L. coprophilum WF2611 与其宿主蛹虫草的关系比 C. cordycipiticola NT1504 更为密切。据我们所知，这是全球范围内首次报道蛹虫草被粪生帚梗霉感染，这将为该疾病的防治提供有用信息，并有助于蛹虫草的工业化种植。
