Li Xiao, Wang Fen, Xu Yanyan, Liu Guijun, Dong Caihong
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
College of Life Science, University of Chinese Academy of Sciences, Beijing 100101, China.
Int J Mol Sci. 2021 Jan 11;22(2):643. doi: 10.3390/ijms22020643.
Hydrophobins are a family of small secreted proteins found exclusively in fungi, and they play various roles in the life cycle. In the present study, genome wide analysis and transcript profiling of the hydrophobin family in , a well-known edible and medicinal mushroom, were studied. The distribution of hydrophobins in ascomycetes with different lifestyles showed that pathogenic fungi had significantly more hydrophobins than saprotrophic fungi, and class II members accounted for the majority. Phylogenetic analysis of hydrophobin proteins from the species of s.l. indicated that there was more variability among the class II members than class I. Only a few hydrophobin-encoding genes evolved by duplication in s.l., which was inconsistent with the important role of gene duplication in basidiomycetes. Different transcript patterns of four hydrophobin-encoding genes during the life cycle indicated the possible different functions for each. The transcripts of , and can respond to light and were related with the photoreceptors. CmQHYD, with four hydrophobin II domains, was first found in , and multi-domain hydrophobins were only distributed in the species of and . These results could be helpful for further function research of hydrophobins and could provide valuable information for the evolution of hydrophobins.
疏水蛋白是一类仅在真菌中发现的小分泌蛋白家族，它们在真菌生命周期中发挥着多种作用。在本研究中，对一种著名的食药用蘑菇中的疏水蛋白家族进行了全基因组分析和转录谱分析。疏水蛋白在不同生活方式的子囊菌中的分布表明，致病真菌的疏水蛋白比腐生真菌显著更多，且II类成员占多数。对广义伞菌属物种的疏水蛋白进行系统发育分析表明，II类成员比I类成员具有更多变异性。在广义伞菌属中，只有少数疏水蛋白编码基因通过复制进化，这与基因复制在担子菌中的重要作用不一致。四个疏水蛋白编码基因在生命周期中的不同转录模式表明每个基因可能具有不同功能。CmHYD、CmAHYD和CmSHYD的转录本可对光作出反应，并与光感受器相关。具有四个疏水蛋白II结构域的CmQHYD首次在香菇中发现，多结构域疏水蛋白仅分布在香菇属和球盖菇属物种中。这些结果有助于进一步研究疏水蛋白的功能，并可为疏水蛋白的进化提供有价值的信息。
