Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, Vienna International Centre, Vienna, Austria.
Institute of Chemical, Environmental and Bioscience Engineering, Vienna University of Technology, Vienna, Austria.
Sci Rep. 2023 Oct 17;13(1):17633. doi: 10.1038/s41598-023-44479-8.
An area-wide integrated pest management strategy with a sterile insect technique (SIT) component requires a radiation source for the sterilisation of male insects. Self-contained gamma irradiators, which were exclusively used in past SIT programmes, are now facing increasing constraints and challenges due to stringent regulations. As a potential alternative, new generation high output X-ray irradiators have been proposed. The feasibility of using X-ray irradiators was assessed by comparing the effects of both gamma- and X-ray irradiators on biological parameters of Glossina palpalis gambiensis (Vanderplank, 1911), that are important for SIT applications. The gamma irradiator Foss Model 812 and two X-ray irradiators, the Rad Source 2400 and the blood irradiator Raycell Mk2 were used. Glossina palpalis gambiensis males were exposed to radiation as pupae. A radiation dose of 110 Gy or above induced more than 97% sterility in females that mated with the irradiated males for all the irradiators. Adult emergence rate, flight propensity, survival and mating performance did not differ between gamma- and X-rays irradiators. These results suggest that irradiating pupae with a dose of 110 Gy is optimal for both gamma-and X-ray irradiators used in this study, to achieve a sterility of approximately 99%. Similar research on other tsetse species could gradually phase out the use of gamma-ray irradiators in favour of X-rays irradiators, especially for smaller SIT programmes.
区域综合虫害管理策略与不育昆虫技术（SIT）组件需要辐射源来对雄性昆虫进行绝育。在过去的 SIT 项目中，自给式伽马辐照器被独家使用，但由于严格的规定，现在面临着越来越多的限制和挑战。作为一种潜在的替代品，新一代高输出 X 射线辐照器已被提出。通过比较伽马和 X 射线辐照器对重要的 SIT 应用的冈比亚舌蝇 Glossina palpalis gambiensis（Vanderplank，1911）的生物学参数的影响，评估了使用 X 射线辐照器的可行性。伽马辐照器 Foss Model 812 和两种 X 射线辐照器，Rad Source 2400 和血液辐照器 Raycell Mk2 被用于研究。冈比亚舌蝇 Glossina palpalis gambiensis 雄性在蛹期暴露于辐射下。对于所有辐照器，110Gy 或更高的辐射剂量会导致与辐照雄性交配的雌性不育率超过 97%。成虫出现率、飞行倾向、生存和交配性能在伽马和 X 射线辐照器之间没有差异。这些结果表明，对于本研究中使用的伽马和 X 射线辐照器，辐照蛹的 110Gy 剂量是最佳的，以达到约 99%的不育率。对其他采采蝇物种的类似研究可以逐步淘汰伽马射线辐照器，转而使用 X 射线辐照器，特别是对于较小的 SIT 项目。
