Institut des Sciences de l'Environnement et du Développement Rural, Université de Dédougou (UDDG), BP 176, Dédougou, Burkina Faso - Insectarium de Bobo-Dioulasso - Campagne d'Éradication de la mouche Tsé-tsé et de la Trypanosomose (IBD-CETT), BP 1087, Bobo-Dioulasso, Burkina Faso.
Insectarium de Bobo-Dioulasso - Campagne d'Éradication de la mouche Tsé-tsé et de la Trypanosomose (IBD-CETT), BP 1087, Bobo-Dioulasso, Burkina Faso - Insitut du Développement Rural (IDR), Université Nazi Boni (UNB), BP 1091, Bobo-Dioulasso, Burkina Faso.
Parasite. 2023;30:8. doi: 10.1051/parasite/2023009. Epub 2023 Mar 31.
African animal trypanosomoses are vector-borne diseases that cause enormous livestock losses in sub-Saharan Africa, with drastic socio-economic impacts. Vector control in the context of an area-wide integrated pest management program with a sterile insect technique component requires the production of high-quality sterile male tsetse flies. In our study, we evaluated the effect of irradiation on the fecundity of Glossina palpalis gambiensis to identify the optimal dose that will induce maximum sterility while maintaining biological performance as much as possible. In addition, male mating performance was evaluated in semi-field cages. The irradiation doses used were 90, 100, 110, 120, 130, 140, and 150 Gy, and untreated males were used as the control. The results showed that pupal production and emergence rates were higher in batches of females that had mated with fertile males than in those that had mated with irradiated males with any experimental dose. A dose of 120 Gy administered to male flies induced 97-99% sterility after mating with virgin females. For the semi-field cage experiments, males irradiated with 120 Gy showed good sexual competitiveness as compared to fertile males and those irradiated with 140 Gy, considering the level of filling of spermatheca and the number of pairs formed. The optimal radiation dose of 120 Gy found in this study is slightly different from the traditional dose of 110 Gy that has been used in several eradication programmes in the past. The potential reasons for this difference are discussed, and an argument is made for the inclusion of reliable dosimetry systems in these types of studies.
非洲动物锥虫病是由媒介传播的疾病，在撒哈拉以南非洲地区导致了巨大的牲畜损失，对社会经济造成了严重影响。在区域综合虫害管理计划的背景下，利用不育昆虫技术进行媒介控制需要生产高质量的不育雄采采蝇。在我们的研究中，我们评估了辐照对冈比亚舌蝇生殖力的影响，以确定最佳剂量，在尽可能保持生物性能的同时诱导最大不育性。此外，还在半野外笼中评估了雄虫的交配性能。使用的辐照剂量分别为 90、100、110、120、130、140 和 150Gy，未处理的雄虫作为对照。结果表明，与用任何实验剂量辐照的雄虫交配的雌虫所产的蛹和羽化率高于与有生育能力的雄虫交配的雌虫。对雄蝇进行 120Gy 的辐照后，与处女雌蝇交配后可诱导 97-99%的不育。对于半野外笼实验，与有生育能力的雄蝇和 140Gy 辐照的雄蝇相比，接受 120Gy 辐照的雄蝇在精包填充水平和形成的对数方面表现出良好的性竞争力。本研究中发现的最佳辐照剂量 120Gy 与过去在几个根除计划中使用的传统剂量 110Gy 略有不同。讨论了产生这种差异的潜在原因，并提出在这类研究中纳入可靠的剂量测定系统。
