来自深海真菌Graphostroma sp. MCCC 3A00421的聚酮化合物具有强大的抗食物过敏活性。

Polyketides from the Deep-Sea-Derived Fungus Graphostroma sp. MCCC 3A00421 Showed Potent Antifood Allergic Activities.

作者信息

Niu Siwen, Liu Qingmei, Xia Jin-Mei, Xie Chun-Lan, Luo Zhu-Hua, Shao Zongze, Liu Guangming, Yang Xian-Wen

机构信息

State Key Laboratory Breeding Base of Marine Genetic Resources, Key Laboratory of Marine Genetic Resources, Fujian Key Laboratory of Marine Genetic Resources, and South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Third Institute of Oceanography, State Oceanic Administration , 184 Daxue Road, Xiamen 361005, China.

College of Food and Biological Engineering, Jimei University , 43 Yindou Road, Xiamen 361021, China.

出版信息

J Agric Food Chem. 2018 Feb 14;66(6):1369-1376. doi: 10.1021/acs.jafc.7b04383. Epub 2018 Feb 2.

DOI:10.1021/acs.jafc.7b04383

PMID:29355320

Abstract

To discover antifood allergic components from deep-sea-derived microorganisms, we performed a systematic chemical investigation of the Atlantic hydrothermal fungus Graphostroma sp. MCCC 3A00421. Consequently, nine new (1-9) and 19 known (10-28) polyketides were isolated. The planar structures of the new compounds were elucidated mainly by detailed analysis of their nuclear magnetic resonance and high-resolution electrospray ionization mass spectrometry data, while the absolute configurations were established using the modified Mosher's method in association with electronic circular dichroism spectra. Graphostrin A (1) is a novel chlorinated polyketide derivate bearing an oxazole moiety. All isolates were tested for antifood allergic bioactivities in immunoglobulin E-mediated rat basophilic leukemia-2H3 cells. Reticulol (10) significantly decreased the rates of degranulation and histamine release with IC values of 13.5 and 13.7 μM, respectively, suggesting reticulol could be a potential antifood allergic medicine.

摘要

为了从深海来源的微生物中发现抗食物过敏成分，我们对大西洋热液真菌Graphostroma sp. MCCC 3A00421进行了系统的化学研究。结果，分离出了9个新的（1-9）和19个已知的（10-28）聚酮化合物。新化合物的平面结构主要通过对其核磁共振和高分辨率电喷雾电离质谱数据的详细分析来阐明，而绝对构型则使用改良的莫舍尔方法结合电子圆二色光谱来确定。Graphostrin A（1）是一种带有恶唑部分的新型氯化聚酮衍生物。所有分离物都在免疫球蛋白E介导的大鼠嗜碱性白血病-2H3细胞中测试了抗食物过敏生物活性。网纹醇（10）显著降低了脱颗粒率和组胺释放率，IC值分别为13.5和13.7 μM，表明网纹醇可能是一种潜在的抗食物过敏药物。