Dental Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Immunol Invest. 2013;42(2):156-63. doi: 10.3109/08820139.2012.746697.
Periodontal disease (PD) and peri-implantitis (PI) are characterized by an immune response leading to destructive inflammation. The prominent impact of genetic factors on periodontitis has been previously evaluated and IL-17 has found to play a critical role in this process. This cytokine has a controversial behavior. This study aimed at finding out whether the polymorphism of this cytokine plays a significant role in chronic periodontitis (CP) and PI or it is just a pro-inflammatory regulatory cytokine. Fresh human blood samples were obtained and three main genotypes were traced carefully. The samples were transferred into 96-well plates and sent to KBioscience Institute in the United Kingdom for genotyping the polymorphism using Competitive Allele Specific PCR (KASP) technique. SPSS version19 software and chi-square and Kruskal-Wallis tests were used for statistical differences considering p-value less than 0.05. A significant difference was detected between the three groups in terms of specific SNP studied in this experiment (P = 0.00). The CC genotype of IL17 polymorphism (rs10484879) may contribute to the pathogenesis of peri-implantitis and periodontitis. The association of IL-17 polymorphism with PI and CP is a promising finding that may help in future similar studies on other ethnicities and larger study populations.
牙周病（PD）和种植体周围炎（PI）的特征是免疫反应导致破坏性炎症。遗传因素对牙周炎的显著影响以前已经得到评估，IL-17 被发现在此过程中起着关键作用。这种细胞因子的行为存在争议。本研究旨在确定这种细胞因子的多态性是否在慢性牙周炎（CP）和 PI 中起重要作用，或者它只是一种促炎调节细胞因子。本研究采集了新鲜的人血样本，并仔细追踪了三种主要基因型。将样本转移到 96 孔板中，并送往英国 KBioscience 研究所，使用竞争性等位基因特异性 PCR（KASP）技术进行多态性基因分型。SPSS 版本 19 软件和卡方检验和 Kruskal-Wallis 检验用于统计差异，考虑到 P 值小于 0.05。在这项实验中研究的特定 SNP 方面，三组之间存在显著差异（P = 0.00）。IL17 多态性（rs10484879）的 CC 基因型可能有助于种植体周围炎和牙周炎的发病机制。IL-17 多态性与 PI 和 CP 的关联是一个有前途的发现，它可能有助于未来对其他种族和更大的研究人群进行类似的研究。
