Colombo Eleonora, Aydın Elif Merve, Canıtez İdil Su, Polito Laura, Penconi Marta, Bossi Alberto, Impresari Elisa, Passarella Daniele, Dallavalle Sabrina, Athanassopoulos Constantinos M, Pellegrino Sara, Şahin Irem Durmaz, Christodoulou Michael S
Dipartimento di Chimica, Universitá degli Studi di Milano, 20133 Milano, Italy.
Ann Romney Center for Neurologic Diseases, Department of Neurology, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts 02115, United States.
ACS Med Chem Lett. 2023 Sep 29;14(10):1472-1477. doi: 10.1021/acsmedchemlett.3c00396. eCollection 2023 Oct 12.
The conjugation of tetraphenylethylene (TPE) with podophyllotoxin, -desacetylthiocolchicine, and cabazitaxel through a sebacic acid linker led to the formation of fluorescent nanoparticles. Dynamic light scattering (DLS) and photoluminescence spectroscopy were used for the identification and characterization of the fluorescent nanoparticles. The biological evaluation was determined in three human ovarian (KURAMOCHI, OVCAR3, OVSAHO) and three human breast (MCF7, SKBR 3, and MDA-MB231) cancer cell lines. In the case of cabazitaxel, the nanoparticles maintained the activity of the parent drug, at the low nanomolar range, while exhibiting high blue fluorescence. The internalization of the fluorescent NPs into cells was detected using immunofluorescence assay.
通过癸二酸连接体将四苯乙烯（TPE）与鬼臼毒素、去乙酰硫代秋水仙碱和卡巴他赛共轭，导致形成荧光纳米颗粒。动态光散射（DLS）和光致发光光谱用于荧光纳米颗粒的鉴定和表征。在三种人卵巢癌细胞系（仓持、OVCAR3、OVSAHO）和三种人乳腺癌细胞系（MCF7、SKBR 3和MDA-MB231）中进行生物学评价。就卡巴他赛而言，纳米颗粒在低纳摩尔范围内保持了母体药物的活性，同时呈现出高蓝色荧光。使用免疫荧光测定法检测荧光纳米颗粒在细胞内的内化情况。
