Infantino Maria, Bizzaro Nicola, de Melo Cruvinel Wilson, Chan Edward K L, Andrade Luis E C
Immunology and Allergy Laboratory, San Giovanni di Dio Hospital, Florence, Italy.
Laboratory of Clinical Pathology, Azienda Sanitaria Universitaria Integrata, Udine, Italy.
Clin Chem Lab Med. 2023 Oct 19;62(5):830-834. doi: 10.1515/cclm-2023-0752. Print 2024 Apr 25.
The indirect immunofluorescence assay on HEp-2 cells (HEp-2 IFA) is still considered the reference method to detect anti-nuclear antibodies (ANA) because of its high sensitivity and represents a relevant tool for the diagnosis of autoimmune rheumatic diseases. During the last decade, the International Consensus on ANA Patterns (ICAP) initiative promoted harmonization and understanding of HEp-2 IFA staining pattern nomenclature, as well as promoting their use in patient care by providing interpretation for HEp-2 IFA test results. In conjunction with a nationwide survey on the evolution of autoantibody diagnostics in autoimmune rheumatic diseases, we focused on the adherence of the Italian laboratories to the ICAP nomenclature analyzing its lights and shadows. The recent ICAP-oriented report, largely used today among Italian laboratories, also represents a further step in harmonizing and improving communication with the clinicians, adding value to laboratory findings and helping with critical clinical decisions.
由于其高灵敏度，人喉表皮样癌细胞间接免疫荧光检测法（HEp-2 IFA）仍被视为检测抗核抗体（ANA）的参考方法，并且是诊断自身免疫性风湿性疾病的重要工具。在过去十年中，抗核抗体模式国际共识（ICAP）倡议促进了对HEp-2 IFA染色模式命名法的统一和理解，并通过提供HEp-2 IFA检测结果的解释来推动其在患者护理中的应用。结合一项关于自身免疫性风湿性疾病自身抗体诊断演变的全国性调查，我们专注于分析意大利实验室对ICAP命名法的遵循情况及其优缺点。最近这份以ICAP为导向的报告如今在意大利实验室中广泛使用，它也是在统一和改善与临床医生沟通方面迈出的又一步，为实验室检查结果增添价值并有助于做出关键的临床决策。
