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来自肝素化脂肪脱细胞基质的脂肪源性生长因子的递送可加速伤口愈合。

Delivery of adipose-derived growth factors from heparinized adipose acellular matrix accelerates wound healing.

作者信息

Ru Jiangjiang, Zhang Qian, Zhu Shaowei, Cai Junrong, He Yunfan, Lu Feng

机构信息

Department of Plastic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.

出版信息

Front Bioeng Biotechnol. 2023 Oct 3;11:1270618. doi: 10.3389/fbioe.2023.1270618. eCollection 2023.

DOI:10.3389/fbioe.2023.1270618
PMID:37854882
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10579818/
Abstract

Dermal white adipocytes are closely associated with skin homeostasis and wound healing. However, it has not been fully investigated whether adipose-derived products improve wound healing. Here, we obtained adipose acellular matrix (AAM) and adipose-derived growth factors (ADGFs) from human adipose tissue and fabricated an ADGF-loaded AAM via surface modification with heparin. The product, HEP-ADGF-AAM, contained an adipose-derived scaffold and released ADGFs in a controlled fashion. To test its efficacy in promoting wound healing, mice with full thickness wound received three different treatments: HEP-ADGF-AAM, AAM and ADM. Control mice received no further treatments. Among these treatments, HEP-ADGF-AAM best improved wound healing. It induced adipogenesis after implantation and provided an adipogenic microenvironment for wounds by releasing ADGFs. HEP-ADGF-AAM not only induced adipocyte regeneration, but also enhanced fibroblast migration, promoted vessel formation, accelerated wound closure, and enhanced wound epithelialization. Moreover, there was a close interaction between HEP-ADGF-AAM and the wound bed, and collagen was turned over in HEP-ADGF-AAM. These results show that HEP-ADGF-AAM might substantially improve re-epithelialization, angiogenesis, and skin appendage regeneration, and is thus a promising therapeutic biomaterial for skin wound healing.

摘要

真皮白色脂肪细胞与皮肤稳态和伤口愈合密切相关。然而，脂肪衍生产物是否能改善伤口愈合尚未得到充分研究。在此，我们从人脂肪组织中获取了脂肪脱细胞基质（AAM）和脂肪衍生生长因子（ADGFs），并通过肝素表面修饰制备了负载ADGFs的AAM。该产物，即HEP-ADGF-AAM，包含脂肪衍生支架并以可控方式释放ADGFs。为测试其促进伤口愈合的功效，对全层伤口小鼠进行了三种不同处理：HEP-ADGF-AAM、AAM和ADM。对照小鼠未接受进一步处理。在这些处理中，HEP-ADGF-AAM对伤口愈合的改善效果最佳。它在植入后诱导脂肪生成，并通过释放ADGFs为伤口提供脂肪生成微环境。HEP-ADGF-AAM不仅诱导脂肪细胞再生，还增强成纤维细胞迁移、促进血管形成、加速伤口闭合并增强伤口上皮化。此外，HEP-ADGF-AAM与伤口床之间存在密切相互作用，且HEP-ADGF-AAM中胶原蛋白发生了更新。这些结果表明，HEP-ADGF-AAM可能显著改善再上皮化、血管生成和皮肤附属器再生，因此是一种有前景的用于皮肤伤口愈合的治疗性生物材料。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84f7/10579818/3f9c8b7f0f4b/fbioe-11-1270618-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84f7/10579818/c2103ee8a081/fbioe-11-1270618-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84f7/10579818/8cb3710472dd/fbioe-11-1270618-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84f7/10579818/05d2808b3376/fbioe-11-1270618-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84f7/10579818/7c7816ace786/fbioe-11-1270618-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84f7/10579818/214a6f5d8769/fbioe-11-1270618-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84f7/10579818/2201f3dfc860/fbioe-11-1270618-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84f7/10579818/3f9c8b7f0f4b/fbioe-11-1270618-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84f7/10579818/c2103ee8a081/fbioe-11-1270618-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84f7/10579818/8cb3710472dd/fbioe-11-1270618-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84f7/10579818/05d2808b3376/fbioe-11-1270618-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84f7/10579818/7c7816ace786/fbioe-11-1270618-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84f7/10579818/214a6f5d8769/fbioe-11-1270618-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84f7/10579818/2201f3dfc860/fbioe-11-1270618-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84f7/10579818/3f9c8b7f0f4b/fbioe-11-1270618-g007.jpg

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