Department of Plastic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Shuaifuyuan 1#, Dongcheng District, Beijing, 100730, China.
Tissue Eng Regen Med. 2020 Dec;17(6):863-874. doi: 10.1007/s13770-020-00286-0. Epub 2020 Nov 9.
Due to adipose-derived stem cells (ASCs) being easy to obtain, their rapid proliferation rate, and their multidirectional differentiation capabilities, they have been widely used in the field of regenerative medicine. With the progress of decellularized adipose tissue (DAT) and adipose tissue engineering research, the role of DAT in promoting angiogenesis has gradually been emphasized.
We examined the biological characteristics and biosafety of DAT and evaluated the stem cell maintenance ability and promotion of growth factor secretion through conducting in vitro and in vivo studies.
The tested ASCs showed high rat:es of proliferation and adhered well to DAT. The expression levels of essential genes for cell stem maintenance, including OCT4, SOX2, and Nanog were low at 2-24 h and much higher at 48 and 96 h. The Adipogenic expression level of markers for ASCs proliferation including PPARγ, C/EPBα, and LPL increased from 2 to 96 h. Co-culture of ASCs and DAT increased the secretion of local growth factors, such as VEGF, PDGF-bb, bFGF, HGF, EGF, and FDGF-bb, and secretion gradually increased from 0 to 48 h. A model of full-thickness skin defects on the back of nude mice was established, and the co-culture of ASCs and DAT showed the best in vivo treatment effect.
The application of DAT promotes wound healing, and DAT combined with ASCs may be a promising material in adipose tissue engineering and regenerative medicine.
脂肪来源干细胞（ASCs）具有获取容易、增殖速度快、多向分化能力强等特点，已广泛应用于再生医学领域。随着脱细胞脂肪组织（DAT）和脂肪组织工程研究的进展，DAT 在促进血管生成中的作用逐渐受到重视。
我们研究了 DAT 的生物学特性和生物安全性，并通过体外和体内研究评估了其维持干细胞能力和促进生长因子分泌的能力。
所检测的 ASCs 增殖率高，对 DAT 黏附良好。细胞干细胞维持的必需基因（包括 OCT4、SOX2 和 Nanog）的表达水平在 2-24 h 时较低，而在 48 和 96 h 时则较高。ASCs 增殖的脂肪生成标志物，如 PPARγ、C/EPBα 和 LPL 的表达水平从 2 h 增加到 96 h。ASCs 和 DAT 的共培养增加了局部生长因子的分泌，如 VEGF、PDGF-bb、bFGF、HGF、EGF 和 FDGF-bb，分泌量从 0 到 48 h 逐渐增加。在裸鼠背部建立全层皮肤缺损模型，发现 ASCs 和 DAT 的共培养具有最佳的体内治疗效果。
DAT 的应用促进了伤口愈合，DAT 与 ASCs 联合应用可能是脂肪组织工程和再生医学中很有前途的材料。
