Wellman Center for Photomedicine and Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
Sci Rep. 2023 Oct 19;13(1):17881. doi: 10.1038/s41598-023-45018-1.
We present a miniature oblique back-illumination microscope (mOBM) for imaging the microcirculation of human oral mucosa, enabling real-time, label-free phase contrast imaging of individual leukocytes circulating in the bloodstream, as well as their rolling and adhesion on vascular walls-the initial steps in leukocyte recruitment that is a hallmark of inflammation. Using the mOBM system, we studied the leukocyte-endothelial interactions in healthy and locally inflamed tissue and observed drastic changes in leukocyte movement (velocity and displacement profile). Our findings suggest that real-time imaging of leukocyte dynamics can provide new diagnostic insights (assessment of inflammation, temporal progression of disease, evaluation of therapeutic response, etc.) that are not available using conventional static parameters such as cell number and morphology.
我们提出了一种微型斜向背照显微镜（mOBM），用于对人口腔黏膜的微循环进行成像，实现了对血流中单个白细胞的实时、无标记的相差对比成像，以及它们在血管壁上的滚动和黏附——这是白细胞募集的初始步骤，也是炎症的标志。使用 mOBM 系统，我们研究了健康组织和局部炎症组织中的白细胞-内皮相互作用，并观察到白细胞运动（速度和位移分布）发生了剧烈变化。我们的研究结果表明，实时成像白细胞动力学可以提供新的诊断见解（评估炎症、疾病的时间进展、治疗反应的评估等），这些见解是使用传统的静态参数（如细胞数量和形态）无法获得的。
