Department of Ophthalmology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan.
Laboratory of Photobiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan.
Sci Rep. 2023 Oct 19;13(1):17861. doi: 10.1038/s41598-023-44983-x.
Myopia is an abnormal vision condition characterized by difficulties in seeing distant objects. Myopia has become a public health issue not only in Asian countries but also in Western countries. Previously, we found that violet light (VL, 360-400 nm wavelength) exposure effectively suppressed myopia progression in experimental chick and mice models of myopia. The inhibitory effects of VL on myopia progression are reduced in retina-specific opsin 5 (Opn5) knockout (KO) mice. Furthermore, VL exposure upregulated early growth response-1 (Egr-1) expression in the chorioretinal tissues of chicks. However, the expression of EGR-1 and role of OPN5 in mice following VL exposure remain unclear. In this study, we examined whether VL exposure-induced EGR-1 upregulation depends on Opn5 expression in the mouse retina. EGR-1 mRNA and protein expressions increased in the mouse retina and mouse retinal 661W cells following VL exposure. These increases were consistently reduced in retina specific Opn5 conditional KO mice and Opn5 KO 661W cells. Our results suggest that OPN5 mediates VL-induced EGR-1 upregulation in mice. These molecular targets could be considered for the prevention and treatment of myopia.
近视是一种异常的视觉状况，其特征是难以看清远处的物体。近视不仅是亚洲国家，也是西方国家的一个公共卫生问题。此前，我们发现，紫光（VL，360-400nm 波长）照射可有效抑制近视鸡和近视小鼠模型的近视进展。VL 对近视进展的抑制作用在视网膜特异性视蛋白 5（Opn5）敲除（KO）小鼠中降低。此外，VL 暴露可上调鸡脉络膜视网膜组织中的早期生长反应因子-1（Egr-1）的表达。然而，VL 暴露后 EGR-1 的表达及其在小鼠中的作用尚不清楚。在本研究中，我们研究了 VL 暴露诱导的 EGR-1 上调是否依赖于小鼠视网膜中的 Opn5 表达。VL 暴露后，小鼠视网膜和小鼠视网膜 661W 细胞中的 EGR-1 mRNA 和蛋白表达增加。在视网膜特异性 Opn5 条件性 KO 小鼠和 Opn5 KO 661W 细胞中，这些增加明显减少。我们的结果表明，Opn5 介导了 VL 诱导的小鼠 EGR-1 上调。这些分子靶标可用于近视的预防和治疗。
