Wang Dongpeng, Dong Yan, Xie Ying, Xiao Yangxin, Ke Chang, Shi Kun, Zhou Zhongshi, Tu Jiyuan, Qu Linghang, Liu Yanju
College of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, China.
Center for Hubei TCM Processing Technology Engineering, Wuhan 430065, China.
J Agric Food Chem. 2023 Oct 20. doi: 10.1021/acs.jafc.3c05173.
Plant-derived polysaccharides, such as rhizome polysaccharide (ALP), are good immune regulators. However, the immune regulatory mechanism of the ALP is unknown. This study aimed to evaluate the effects of ALP on the intestinal mucosal barrier and intestinal mucosal immunity of immunosuppressed mice. We also compared the activity of raw rhizome polysaccharide (SALP) with wheat bran processed bran-fried rhizome polysaccharide (FALP; both at 1.2 g/kg/d for mice). Our results showed that ALP effectively increased the immune organ index and blood cell count, stimulated the secretion of cytokines, and promoted the expression of occludin and zonula occludens-1 (ZO-1). ALP also promoted the expression of T cells and the secretion of sIgA. Furthermore, ALP alleviated the gut microbiota disorder in Cy-treated mice and increased the relative abundances of and . ALP reversed the decrease in the level of SCFAs and promoted the expression of G protein-coupled receptor 43 (GPR43). To our knowledge, this study was the first to explore how the ALP protects the intestinal mucosal barrier and enhances intestinal mucosal immunity by alleviating the gut microbiota imbalance and metabolic disorders of SCFAs. FALP was more therapeutic than SALP, suggesting that FALP could be developed as a promising functional food component.
植物来源的多糖，如根茎多糖（ALP），是良好的免疫调节剂。然而，ALP的免疫调节机制尚不清楚。本研究旨在评估ALP对免疫抑制小鼠肠道黏膜屏障和肠道黏膜免疫的影响。我们还比较了生根茎多糖（SALP）与经麦麸炮制的麸炒根茎多糖（FALP；小鼠均为1.2 g/kg/d）的活性。我们的结果表明，ALP有效地增加了免疫器官指数和血细胞计数，刺激了细胞因子的分泌，并促进了闭合蛋白和紧密连接蛋白1（ZO-1）的表达。ALP还促进了T细胞的表达和分泌型免疫球蛋白A（sIgA）的分泌。此外，ALP减轻了环磷酰胺处理小鼠的肠道微生物群紊乱，并增加了[具体微生物名称1]和[具体微生物名称2]的相对丰度。ALP逆转了短链脂肪酸（SCFAs）水平的下降，并促进了G蛋白偶联受体43（GPR43）的表达。据我们所知，本研究首次探讨了ALP如何通过减轻肠道微生物群失衡和SCFAs代谢紊乱来保护肠道黏膜屏障并增强肠道黏膜免疫。FALP比SALP具有更强的治疗效果，表明FALP有望开发成为一种功能性食品成分。
