Xu Di, Wu Mengmeng, Gao Zenghao, Zhao Yue, Hu Meng, Wen Yang, Wang Linlin, Xu Deli, Chen Lei
College of Life Sciences Qufu Normal University Qufu China.
Shandong Freshwater Fisheries Research Institute Jinan China.
Ecol Evol. 2025 May 7;15(5):e71382. doi: 10.1002/ece3.71382. eCollection 2025 May.
The composition of animal gut microbiota is significantly affected by a variety of factors. Seasonal variation in environmental factors is believed to have a significant impact on the composition of mammalian gut microbiota. Therefore, studying the seasonal differences in gut microbiota diversity in wildlife is of great importance to explore their ecological adaptability. This study compared the diversity of gut microbiota of the short-faced moles () in spring, summer, and autumn by using 16S rRNA amplification sequencing. Our results reveal significant seasonal differences in the diversity and function of the short-faced moles gut microbiota. Compared to spring, the diversity and function of the gut microbiota in summer and autumn of short-faced moles are more similar to each other. The relative abundance of Firmicutes is higher in spring than in summer and autumn, while the relative abundance of Proteobacteria in summer and autumn is higher than that of spring. There are significant differences in carbohydrate metabolism between spring and summer, and between spring and autumn. The correlation analysis results suggest that climatic factors are strongly associated with seasonal variation in gut microbiota of the short-faced moles, especially temperature and relative humidity. The present study discusses the seasonal variations in the gut microbiota diversity of short-faced moles and the significant impact of climatic factors on gut microbiota diversity. These results will highlight the potential impact of climatic factors on the seasonal changes of the gut microbiota of subterranean mammals and provide a new view for comprehensively understanding the ecological adaptation of subterranean mammals.
动物肠道微生物群的组成受到多种因素的显著影响。环境因素的季节性变化被认为对哺乳动物肠道微生物群的组成有重大影响。因此，研究野生动物肠道微生物群多样性的季节性差异对于探索它们的生态适应性具有重要意义。本研究通过16S rRNA扩增测序比较了短脸鼹鼠在春季、夏季和秋季的肠道微生物群多样性。我们的结果揭示了短脸鼹鼠肠道微生物群在多样性和功能上存在显著的季节性差异。与春季相比，短脸鼹鼠夏季和秋季肠道微生物群的多样性和功能彼此更为相似。厚壁菌门在春季的相对丰度高于夏季和秋季，而变形菌门在夏季和秋季的相对丰度高于春季。春季与夏季之间以及春季与秋季之间在碳水化合物代谢方面存在显著差异。相关性分析结果表明，气候因素与短脸鼹鼠肠道微生物群的季节性变化密切相关，尤其是温度和相对湿度。本研究讨论了短脸鼹鼠肠道微生物群多样性的季节性变化以及气候因素对肠道微生物群多样性的重大影响。这些结果将突出气候因素对地下哺乳动物肠道微生物群季节性变化的潜在影响，并为全面理解地下哺乳动物的生态适应性提供新的视角。
