Department of Basic Medical Sciences, College of Medicine, QU Health, Qatar University, Doha, Qatar.
Department of Biomedical Sciences College of Medicine, QU Health, Qatar University, Doha, Qatar.
J Nutr Biochem. 2024 Jan;123:109472. doi: 10.1016/j.jnutbio.2023.109472. Epub 2023 Oct 19.
Vitamin D deficiency and dyslipidemia have substantial implications for human health globally. Vitamin D is essential for bone metabolism and immune modulation, and its insufficiency is linked to various chronic inflammatory conditions. Dyslipidemia, characterized by low levels of high-density lipoprotein (HDL) and elevated levels of low-density lipoprotein (LDL) and triglycerides, is also prevalent. Previous research has shown a connection between vitamin D deficiency and low HDL, but the precise mechanism by which vitamin D influences HDL production and its anti-inflammatory properties remains unclear. This study aimed to investigate the proteomic profiles of individuals with and without vitamin D deficiency and dyslipidemia, specifically focusing on the effects of vitamin D on HDL production, its anti-inflammatory potential, and the molecular pathways associated with vitamin D deficiency and dyslipidemia, particularly inflammation and cancer pathways. By analyzing the proteomic profiles of 274 participants from the Qatar Biobank database, we identified 1301 proteins. Our findings indicated a decrease in HDL-associated apolipoproteins (ApoM and ApoD) in individuals with both dyslipidemia and vitamin D deficiency. Conversely, participants with these conditions exhibited increased expression of acute-phase proteins (SAA1 and SOD1), which are associated with inflammation. Pathway enrichment analysis revealed heightened inflammatory activity in individuals with vitamin D deficiency and dyslipidemia, with notable enrichments in pathways such as MAPK, JAK-STAT, Ras signaling, cytokine-cytokine receptor interaction, AGE-RAGE, ErbB signaling, and cancer pathways. Overall, cases of vitamin D deficiency showed enrichment in inflammation pathways, while individuals with both vitamin D deficiency and dyslipidemia demonstrated enhanced activation of cancer and inflammation pathways.
维生素 D 缺乏和血脂异常对全球人类健康有重大影响。维生素 D 对骨骼代谢和免疫调节至关重要，其不足与各种慢性炎症状态有关。血脂异常的特征是高密度脂蛋白 (HDL) 水平低，低密度脂蛋白 (LDL) 和甘油三酯水平高，也很常见。先前的研究表明，维生素 D 缺乏与低 HDL 之间存在关联，但维生素 D 影响 HDL 生成及其抗炎特性的确切机制尚不清楚。本研究旨在研究维生素 D 缺乏和血脂异常个体的蛋白质组谱，特别是关注维生素 D 对 HDL 生成、其抗炎潜力以及与维生素 D 缺乏和血脂异常相关的分子途径（特别是炎症和癌症途径）的影响。通过分析来自卡塔尔生物银行数据库的 274 名参与者的蛋白质组谱，我们鉴定出 1301 种蛋白质。我们的研究结果表明，在同时患有血脂异常和维生素 D 缺乏的个体中，与 HDL 相关的载脂蛋白 (ApoM 和 ApoD) 减少。相反，这些患者表现出急性期蛋白 (SAA1 和 SOD1) 的表达增加，这与炎症有关。途径富集分析显示，维生素 D 缺乏和血脂异常患者的炎症活性增强，MAPK、JAK-STAT、Ras 信号、细胞因子-细胞因子受体相互作用、AGE-RAGE、ErbB 信号和癌症途径等途径显著富集。总的来说，维生素 D 缺乏症病例表现出炎症途径的富集，而同时患有维生素 D 缺乏症和血脂异常的个体则表现出癌症和炎症途径的增强激活。
