Miljkovic Milica, Stefanovic Aleksandra, Simic-Ogrizovic Sanja, Vekic Jelena, Bogavac-Stanojevic Natasa, Cerne Darko, Kocbek Petra, Marc Janja, Jelic-Ivanovic Zorana, Spasojevic-Kalimanovska Vesna, Kotur-Stevuljevic Jelena
1 Department of Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia.
2 Clinics of Nephrology, Clinical Center of Serbia, Belgrade, Serbia.
Angiology. 2018 Nov;69(10):861-870. doi: 10.1177/0003319718780041. Epub 2018 Jun 18.
Some cardiovascular complications in patients with chronic kidney disease and end-stage renal disease may be caused by structurally and functionally modified lipoproteins. Redox status (advanced oxidation protein products [AOPPs]), prooxidant-antioxidant balance, total protein sulfhydryl (SH-groups), and paraoxonase 1 (PON1) activity were assessed in 77 renal patients and 20 controls. Lipoproteins were isolated using ultracentrifugation. PON1, PON3, and pentraxin-3 concentration were determined by enzyme-linked immunosorbent assay (ELISA). Dyslipidemia-Oxy-Inflammation (DOI) score was calculated as a sum of dyslipidemia, oxidative stress, and inflammation scores. The dyslipidemia score ( P < .001), oxy score ( P < .01), inflammation score (P < .001), and the DOI score ( P < .001) were higher in patient groups compared with controls. The very-low-density lipoprotein (VLDL) fraction contained the highest amount of AOPP ( P < .001) compared with other lipoprotein fractions in all groups. The low-density lipoprotein (LDL) fraction contained elevated AOPP in all groups compared with the high-density lipoprotein (HDL) fraction ( P < .001). Significant positive correlation was observed between AOPP in LDL fraction and DOI score (ρ = 0.510, P < .01). Dyslipidemia, oxidative stress, and inflammation play an interactive role in renal disease and are mutually associated with redox status in VLDL, LDL, and HDL lipoproteins in plasma of renal patients.
慢性肾脏病和终末期肾病患者的一些心血管并发症可能由结构和功能发生改变的脂蛋白引起。对77例肾病患者和20例对照者评估了氧化还原状态（晚期氧化蛋白产物[AOPPs]）、促氧化剂-抗氧化剂平衡、总蛋白巯基（SH基团）和对氧磷酶1（PON1）活性。采用超速离心法分离脂蛋白。通过酶联免疫吸附测定（ELISA）测定PON1、PON3和五聚素3浓度。计算血脂异常-氧化-炎症（DOI）评分，即血脂异常、氧化应激和炎症评分之和。与对照组相比，患者组的血脂异常评分（P <.001）、氧化评分（P <.01）、炎症评分（P <.001）和DOI评分（P <.001）更高。与所有组的其他脂蛋白组分相比，极低密度脂蛋白（VLDL）组分中的AOPP含量最高（P <.001）。与高密度脂蛋白（HDL）组分相比，所有组的低密度脂蛋白（LDL）组分中的AOPP含量均升高（P <.001）。LDL组分中的AOPP与DOI评分之间存在显著正相关（ρ = 0.510，P <.01）。血脂异常、氧化应激和炎症在肾病中起相互作用，并且与肾病患者血浆中VLDL、LDL和HDL脂蛋白的氧化还原状态相互关联。
