Tashakorizadeh Mansoureh, Golkar Pooran, Vahabi Mohammad Reza, Ghorbanpour Mansour
Forests and Rangelands Research Department, Kerman Agricultural and Natural Resources Research and Education Center, Agricultural Research Education and Extension Organization (AREEO), Kerman, Iran.
Department of Natural Resources, Isfahan University of Technology, Isfahan, 8415683111, Iran.
Sci Rep. 2023 Oct 20;13(1):17934. doi: 10.1038/s41598-023-45103-5.
Soil contamination with heavy metals adversely affects plants growth, development and metabolism in many parts of the world including arid and semi-arid regions. The aim of this study was to investigate the single and combined effects of drought and copper (Cu) stresses on seed yield, and biochemical traits of Fumaria parviflora in a split - factorial experiment at Research Field of Payam-E-Noor university of Kerman during 2019. The collected seeds from two Cu contaminated regions were evaluated under drought and Cu (0, 50, 150, 300, and 400 mg/kg) stresses. Drought stress levels were depletion of 50% (D), 70% (D) and 85% (D) soil available water. The individual effects of drought and copper stresses were similar to each other as both reduced seed yield. The highest seed yield was observed at Cu concentration of 50 mg/kg under non-drought stress conditions. The maximum values of malondialdehyde (0.47 µmol/g), proline (2.45 µmol/g FW), total phenolics (188.99 mg GAE/g DW) and total flavonoids (22.1 mg QE/g DW) were observed at 400 mg/kg Cu treatment. However, the strongest antioxidant activity (83.95%) through DPPH assay, and the highest total soluble carbohydrate (115.23 mg/g DW) content were observed at 300 and 150 mg/kg Cu concentration under severe drought stress, respectively. The highest amount of anthocyanin (2.18 µmol/g FW) was observed at 300 mg/kg Cu and moderate drought stress. The findings of this study showed a high tolerance of F. parviflora plant to moderate drought stress and Cu exposure up to 150 mg/kg by modulating defense mechanisms, where grain yield was slightly lower than that of control. The results could also provide a criterion for the selection of tolerance species like F. parviflora for better acclimatization under Cu mines and/or agricultural contaminated soils subjected to drought stress.
在世界许多地区，包括干旱和半干旱地区，土壤重金属污染对植物的生长、发育和新陈代谢产生不利影响。本研究的目的是在2019年克尔曼佩亚姆 - 努尔大学研究田进行的裂区试验中，研究干旱和铜（Cu）胁迫对小花烟堇种子产量和生化特性的单一和联合影响。从两个铜污染地区收集的种子在干旱和铜（0、50、150、300和400 mg/kg）胁迫下进行评估。干旱胁迫水平为土壤有效水分消耗50%（D50）、70%（D70）和85%（D85）。干旱和铜胁迫的个体效应彼此相似，因为两者都降低了种子产量。在非干旱胁迫条件下，铜浓度为50 mg/kg时观察到最高种子产量。在400 mg/kg铜处理下，丙二醛（0.47 µmol/g）、脯氨酸（2.45 µmol/g FW）、总酚（188.99 mg GAE/g DW）和总黄酮（22.1 mg QE/g DW）的最大值被观察到。然而，通过DPPH测定法，最强的抗氧化活性（83.95%）以及在严重干旱胁迫下，分别在300和150 mg/kg铜浓度下观察到最高的总可溶性碳水化合物（115.23 mg/g DW）含量。在300 mg/kg铜和中度干旱胁迫下，花青素（2.18 µmol/g FW）含量最高。本研究结果表明，小花烟堇植物通过调节防御机制对中度干旱胁迫和高达150 mg/kg的铜暴露具有较高的耐受性，其籽粒产量略低于对照。这些结果还可为选择像小花烟堇这样的耐性物种提供标准，以便在铜矿和/或遭受干旱胁迫的农业污染土壤中更好地适应环境。
