Department of Biomaterials, Faculty of Interdisciplinary Science and Technology, Tarbiat Modares University, 14115-336 Tehran, Iran.
Department of Biomaterials, Faculty of Interdisciplinary Science and Technology, Tarbiat Modares University, 14115-336 Tehran, Iran; Department of Nanobiotechnalogy, Faculty of Biological Sciences, Tarbiat Modares University, 14115-154 Tehran, Iran.
J Dent. 2023 Dec;139:104749. doi: 10.1016/j.jdent.2023.104749. Epub 2023 Oct 20.
The effectiveness of three different groups of polyethylene glycol (PEG)-based gels containing powders on dentin hypersensitivity (DH) treatment were assessed and compared with Actimins® as commercial reference group.
Hydroxyapatite nanorods (nHA) and sol-gel-derived 45S5 bioglass (SGD 45S5) powders were synthesized through hydrothermal and sol-gel methods, respectively. First, 25 demineralized dentin disks were divided into five groups. Then, the prepared gels based on 45S5 bioglass with and without hydrolyzed casein (HC) as experimental, nHA gel and Actimins® as positive and commercial reference groups were applied twice a day on disks by a micro applicator. To mimic the oral environment, treated disks were immersed in artificial saliva in a water bath at 37 °C for 7 days. However, in the negative control group, no agent was applied on the samples. FE-SEM, EDS, AFM, and XRD were performed to assess tubule occlusion. One-way ANOVA test was used for statistical analysis and p*<0.05 was set as the significance level.
The nHA with an average aspect ratio of 2.77 and the SGD 45S5 powders with a polygonal morphology and the average size of 48.64±11.38 µm were synthesized. After treatment, tubule occlusion in HC-SGD 45S5 and nHA gels were shown to be higher than other groups. The root mean square roughness (R) of the above-mentioned gels showed to be 121.54±9.25 nm, and 312.6 ± 9 nm, respectively.
The nHA containing group exhibited the highest tubule occlusion efficiency (i.e., tubule diameter of 0.92±0.32 µm) with a superior mineral precipitation. HC as a novel material demonstrates to be potentially beneficial in DH treatment.
DH as a common issue may be reduced or eliminated by occlusion of patent dentinal tubules. There are various types of desensitizing agents capable of controlling the DH by the occlusion of patent dentinal tubules. The desensitizing gels developed in this study showed to be promising for clinical and home-use applications.
评估并比较三种不同的基于聚乙二醇（PEG）的凝胶（含粉末）在治疗牙本质敏感症（DH）方面的效果，其中 Actimins®凝胶作为商业参考组。
通过水热法和溶胶-凝胶法分别合成了羟基磷灰石纳米棒（nHA）和溶胶-凝胶衍生的 45S5 生物玻璃（SGD 45S5）粉末。首先，将 25 个脱矿化牙本质圆盘分为五组。然后，将基于 45S5 生物玻璃的凝胶（含水解酪蛋白[HC]和不含水解酪蛋白）、nHA 凝胶和 Actimins®凝胶分别作为实验组、阳性对照组和商业参考组，通过微涂抹器每天在圆盘上涂抹两次。为了模拟口腔环境，将处理后的圆盘在 37°C 的水浴中浸泡在人工唾液中 7 天。然而，在阴性对照组中，样品上不应用任何制剂。采用 FE-SEM、EDS、AFM 和 XRD 评估小管阻塞。采用单因素方差分析进行统计学分析，p*<0.05 为显著性水平。
合成了平均纵横比为 2.77 的 nHA 和具有多边形形态且平均尺寸为 48.64±11.38 µm 的 SGD 45S5 粉末。治疗后，HC-SGD 45S5 和 nHA 凝胶的小管阻塞率高于其他组。上述凝胶的均方根粗糙度（R）分别为 121.54±9.25 nm 和 312.6±9 nm。
含 nHA 的组表现出最高的小管阻塞效率（即小管直径为 0.92±0.32 µm），并具有优异的矿化沉淀。HC 作为一种新型材料，在 DH 治疗中可能具有潜在的益处。
DH 作为一种常见问题，可以通过阻塞开放的牙本质小管来减少或消除。有各种类型的脱敏剂可以通过阻塞开放的牙本质小管来控制 DH。本研究开发的脱敏凝胶具有在临床和家庭应用方面的应用潜力。
