Department of Orthodontics, Dental Research Institute, Pusan National University Dental Hospital, Geumoro 20, Mulgeum, Yangsan, 50612, Republic of Korea.
R&D Center, Upex. Med Co., Ltd., Hagui-ro 282, Dongan-gu, Anyang, Gyeonggi-do, 14056, Republic of Korea.
Clin Oral Investig. 2019 Jan;23(1):253-266. doi: 10.1007/s00784-018-2432-z. Epub 2018 Apr 6.
To synthesize a silver-doped bioactive glass/mesoporous silica nanoparticle (Ag-BGN@MSN), as well as to investigate its effects on dentinal tubule occlusion, microtensile bond strength (MTBS), and antibacterial activity.
Ag-BGN@MSN was synthesized using a modified "quick alkali-mediated sol-gel" method. Demineralized tooth disc models were made and divided into four groups; the following treatments were then applied: group 1-no treatment, group 2-bioglass, group 3-MSN, group 4-Ag-BGN@MSN. Next, four discs were selected from each group and soaked into 6 wt% citric acid to test acid-resistant stability. Dentinal tubule occlusion, as well as the occlusion ratio, was observed using field-emission scanning electron microscopy. The MTBS was also measured to evaluate the desensitizing effect of the treatments. Cytotoxicity was examined using the MTT assay. Antibacterial activity was detected against Lactobacillus casei, and ion dissolution was evaluated using inductively coupled plasma optical emission spectrometry.
Ag-BGN@MSN effectively occluded the dentinal tubule and formed a membrane-like layer. After the acid challenge, Ag-BGN@MSN had the highest rate of dentinal tubule occlusion. There were no significant differences in MTBS among the four groups (P > 0.05). All concentrations of Ag-BGN@MSN used had a relative cell viability above 72%.
Ag-BGN@MSN was successfully fabricated using a modified sol-gel method. The Ag-BGN@MSN biocomposite effectively occluded dentinal with acid-resistant stability, did not decrease bond strength in self-etch adhesive system, had low cytotoxicity, and antibacterial effect.
Dentinal tubule sealing induced by Ag-BGN@MSN biocomposite with antibacterial effect is likely to increase long-term stability in DH.
合成载银生物活性玻璃/介孔硅纳米粒子（Ag-BGN@MSN），并研究其对牙本质小管封闭、微拉伸粘结强度（MTBS）和抗菌活性的影响。
采用改良的“快速碱介导溶胶-凝胶”法合成 Ag-BGN@MSN。制作脱矿牙盘模型并分为四组；然后进行以下处理：第 1 组-无处理，第 2 组-生物玻璃，第 3 组-MS，第 4 组-Ag-BGN@MSN。然后，从每组中选择四个圆盘并浸泡在 6wt%柠檬酸中，以测试耐酸性稳定性。使用场发射扫描电子显微镜观察牙本质小管封闭情况和封闭率。通过 MTBS 测量评估处理的脱敏效果。使用 MTT 测定法检测细胞毒性。检测对干酪乳杆菌的抗菌活性，并使用电感耦合等离子体发射光谱法评估离子溶解。
Ag-BGN@MSN 有效地封闭了牙本质小管并形成了膜状层。经过酸挑战后，Ag-BGN@MSN 的牙本质小管封闭率最高。四组之间的 MTBS 没有显著差异（P＞0.05）。Ag-BGN@MSN 的所有使用浓度的相对细胞活力均高于 72%。
采用改良的溶胶-凝胶法成功制备了 Ag-BGN@MSN。Ag-BGN@MSN 生物复合材料有效封闭牙本质，具有耐酸性稳定性，不会降低自酸蚀粘结系统的粘结强度，具有低细胞毒性和抗菌作用。
具有抗菌作用的 Ag-BGN@MSN 生物复合材料诱导的牙本质小管封闭可能会增加 DH 的长期稳定性。
