School of Public Health, Wuhan University, 430071, China.
Hubei Provincial Center for Disease Control and Prevention, 430079, China.
Int J Biol Macromol. 2023 Dec 31;253(Pt 8):127557. doi: 10.1016/j.ijbiomac.2023.127557. Epub 2023 Oct 20.
In the work, zein-yeast carboxymethyl glucan (ZY) particles were fabricated by a novel ultrasonic assisted anti-solvent precipitation (ASP) method, which was a good delivery system for resveratrol. The particle size and zeta-potential of ZY samples were detected by Zetasizer Pro analyzer, they gradually increased as the mass ratio of zein and yeast carboxymethyl glucan (YCG) changed from 10:1 to 10:5. The intermolecular interactions were investigated by zeta-potentiometric analyzer, Fourier transform infrared spectroscopy and fluorescence spectroscopy. Electrostatic interaction, hydrogen bonding and hydrophobic effects between zein and YCG molecules were identified as the main driving forces in the formation of ZY particles. The optimized ZY (10:3) binary particles were used as delivery system for encapsulating and protecting resveratrol. They had high encapsulation efficiency (85.4 %) and loading capacity (6.1 %), and increased the retention rate of resveratrol by 2.10 and 1.21 folds after exposure to light and heat conditions, effectively protect resveratrol against light and thermal degradation. These particles also delayed the release of resveratrol in simulated gastrointestinal digestion, which might improve its oral bioavailability. In conclusion, ZY binary particles could be regarded as a useful and promising delivery vehicle, which might contribute to the application of hydrophobic bioactive ingredients in functional foods.
在这项工作中，通过一种新颖的超声辅助反溶剂沉淀（ASP）方法制备了玉米醇溶蛋白-酵母羧甲基葡聚糖（ZY）颗粒，这是一种用于白藜芦醇的良好递送系统。通过 Zetasizer Pro 分析仪检测 ZY 样品的粒径和 Zeta 电位，随着玉米醇溶蛋白和酵母羧甲基葡聚糖（YCG）的质量比从 10:1 变为 10:5，它们逐渐增加。通过动电位分析仪、傅里叶变换红外光谱和荧光光谱研究了分子间相互作用。静电相互作用、氢键和玉米醇溶蛋白和 YCG 分子之间的疏水相互作用被确定为形成 ZY 颗粒的主要驱动力。优化的 ZY（10:3）二元颗粒用作封装和保护白藜芦醇的递送系统。它们具有高包封效率（85.4%）和载药量（6.1%），并且在暴露于光照和热条件后，将白藜芦醇的保留率提高了 2.10 和 1.21 倍，有效保护白藜芦醇免受光和热降解。这些颗粒还延迟了模拟胃肠道消化中白藜芦醇的释放，这可能提高其口服生物利用度。总之，ZY 二元颗粒可以被视为一种有用且有前途的递送载体，这可能有助于疏水性生物活性成分在功能性食品中的应用。
