School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China; School of Food Science and Technology, South China Agricultural University, Guangzhou 510642, China.
School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China.
Int J Biol Macromol. 2024 Oct;278(Pt 2):134827. doi: 10.1016/j.ijbiomac.2024.134827. Epub 2024 Aug 20.
Three nanoparticles were fabricated for the co-delivery of quercetin and resveratrol. Nanoparticles consisted of a zein and carboxymethyl cellulose assembled using antisolvent precipitation/layer-by-layer deposition method. Nanoparticles contained quercetin in the core and resveratrol in the shell, resveratrol in the core and quercetin in the shell or both quercetin and resveratrol in the core. The particle sizes of nanoparticles were 280.4, 214.8, and 181.8 nm, respectively. Zeta-potential was about -50 mV and PDI was about 0.3. The different positions of polyphenol distribution nanoparticles could reduce the competition between the two polyphenols, the encapsulation rate, loading rate and storage stability reached up to 91.7 %, 5.37 % and 97.1 %, respectively. FT-IR showed that hydrophobic and electrostatic interactions were the main driving forces of nanoparticle assembly. XRD showed that two polyphenols were successfully encapsulated in nanoparticles. TGA showed that distributing the nanoparticles in different layers would enhance thermal stability. TEM and SEM showed that polysaccharides attached to the surface of nanoparticles formed a core-shell structure with uniform particle size. All three nanoparticles could release two polyphenols slowly in simulated gastrointestinal digestion, Korsmeyer-Peppas was the most suitable kinetic release model. Therefore, biopolymer-based nanocarriers can be created to enhance the loading, stability, and bioaccessibility of co-encapsulated nutraceuticals.
三种纳米颗粒被制备用于槲皮素和白藜芦醇的共递送。纳米颗粒由使用反溶剂沉淀/层层沉积方法组装的玉米醇溶蛋白和羧甲基纤维素组成。纳米颗粒的核含有槲皮素，壳含有白藜芦醇，核含有白藜芦醇，壳含有槲皮素，或者核中同时含有槲皮素和白藜芦醇。纳米颗粒的粒径分别为 280.4、214.8 和 181.8nm。Zeta 电位约为-50mV，PDI 约为 0.3。多酚分布纳米颗粒的不同位置可以减少两种多酚之间的竞争，包封率、载药量和储存稳定性分别达到 91.7%、5.37%和 97.1%。FT-IR 表明疏水性和静电相互作用是纳米颗粒组装的主要驱动力。XRD 表明两种多酚已成功包封在纳米颗粒中。TGA 表明将纳米颗粒分布在不同层中会增强热稳定性。TEM 和 SEM 表明多糖附着在纳米颗粒表面形成具有均匀粒径的核壳结构。三种纳米颗粒都可以在模拟胃肠道消化中缓慢释放两种多酚，Korsmeyer-Peppas 是最适合的动力学释放模型。因此，可以创建基于生物聚合物的纳米载体来增强共包封的营养保健品的负载、稳定性和生物利用度。
