Department of Chemistry, Technology and Biotechnology of Food, Faculty of Chemistry, Gdansk University of Technology, 11/12 G. Narutowicza Street, 80-233, Gdansk, Poland.
Department of Pharmaceutical Technology and Biochemistry, Faculty of Chemistry, Gdansk University of Technology, 11/12 G. Narutowicza Street, 80-233, Gdansk, Poland.
Carbohydr Res. 2023 Dec;534:108973. doi: 10.1016/j.carres.2023.108973. Epub 2023 Oct 14.
This paper presents a comparison of the antimicrobial activity and cytotoxicity against L929 cells of chitosan xerogels prepared by dissolving the polymer in a solution of lactic acid (LA) or carbonic acid (CO) and then freeze-drying. There was no simple relationship between the antimicrobial activity and cytotoxicity of the samples obtained using both techniques (LA and CO). Chitosan materials obtained by the LA method in a 1:1 dilution were characterized by the highest cytotoxicity against L929 cells (∼20%). For the same diluted samples prepared using the CO saturation method, the viability of L929 cells was approximately 2.5 times greater. Some of the tested chitosan materials obtained by the innovative method were characterized by significantly lower antimicrobial activity, for example, reduction of E. coli bacteria for MMW-LA and MMW-CO samples by 6.00 and 0.75 logarithmic order, respectively. This clearly indicates that in many applications, the presence of the acid necessary to dissolve chitosan is responsible for the antimicrobial activity of the polymer solution and its products.
本文比较了通过在乳酸（LA）或碳酸（CO）溶液中溶解聚合物然后冷冻干燥制备的壳聚糖干凝胶的抗菌活性和对 L929 细胞的细胞毒性。使用这两种技术（LA 和 CO）获得的样品的抗菌活性和细胞毒性之间没有简单的关系。在 1:1 稀释的 LA 法中获得的壳聚糖材料对 L929 细胞的细胞毒性最高（约 20%）。对于使用 CO 饱和法制备的相同稀释样品，L929 细胞的活力大约高出 2.5 倍。用创新方法获得的一些测试壳聚糖材料的抗菌活性明显降低，例如，MWM-LA 和 MMW-CO 样品对大肠杆菌的减少分别为 6.00 和 0.75 对数级。这清楚地表明，在许多应用中，溶解壳聚糖所需的酸是聚合物溶液及其产物抗菌活性的原因。
