Department of Analytical Chemistry, Faculty of Pharmacy, Tanta University, Tanta, Egypt.
Molecular Microspectroscopy Laboratory, Miami University, Oxford, USA.
Spectrochim Acta A Mol Biomol Spectrosc. 2024 Jan 15;305:123561. doi: 10.1016/j.saa.2023.123561. Epub 2023 Oct 18.
Examining intact kidney stones both qualitatively and quantitatively can be difficult due to their size and fragility. Many modern analysis methods often lead to the destruction of the stone's structure during sample preparation. Preserving the structural integrity is crucial for accurately determining the chemical distribution of the components of kidney stones, which, in turn, improves our understanding of the disease's etiology. Infrared microspectroscopy and imaging play a vital role in revealing the stone's microstructure and component distribution. Consequently, this research focuses on investigating the impact of different sample preparation techniques on kidney stone analysis using infrared microspectroscopy. Specifically, it explores how polishing the surface of cross-sectioned stones influences the results. The polishing was performed utilizing abrasive discs and lapping films. A polishing device was also designed for the optimization of sample preparation. Additionally, this work involved a comparison of reflection infrared imaging with Attenuated Total Internal Reflection (ATR) infrared microspectroscopic imaging for the analysis of the microstructure of urinary stones.
由于肾结石的大小和易碎性，对其进行定性和定量检查可能很困难。许多现代分析方法往往会在样品制备过程中导致结石结构的破坏。保留结构完整性对于准确确定肾结石成分的化学分布至关重要，而这反过来又可以提高我们对疾病病因的理解。红外显微光谱和成像在揭示结石的微观结构和成分分布方面发挥着重要作用。因此，本研究侧重于研究不同的样品制备技术对使用红外显微光谱进行肾结石分析的影响。具体来说，研究探讨了对结石截面进行表面抛光对结果的影响。使用研磨盘和研磨薄膜进行了抛光。还设计了一种抛光装置来优化样品制备。此外，这项工作还比较了反射红外成像与衰减全内反射（ATR）红外显微光谱成像在分析尿结石微观结构方面的应用。
