基于体外消化和广泛靶向代谢组学分析不同采收期坛紫菜多酚的降血糖活性

Effect of Porphyra haitanensis polyphenols from different harvest periods on hypoglycaemic activity based on in vitro digestion and widely targeted metabolomic analysis.

作者信息

Chen Xiaochen, Xu Yating, Du Xiping, Li Zhipeng, Yang Yuanfan, Jiang Zedong, Ni Hui, Li Qingbiao

机构信息

College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China.

College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China; Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen, Fujian 361021, China; Research Center of Food Biotechnology of Xiamen City, Xiamen, Fujian 361021, China; Key Laboratory of Systemic Utilization and In-depth Processing of Economic Seaweed, Xiamen Southern Ocean Technology Center of China, Xiamen, Fujian 361021, China.

出版信息

Food Chem. 2024 Mar 30;437(Pt 1):137793. doi: 10.1016/j.foodchem.2023.137793. Epub 2023 Oct 18.

DOI:10.1016/j.foodchem.2023.137793

PMID:37866341

Abstract

The hypoglycemic effect of Porphyra is well known. Based on in vitro digestion and metabolomics, the bioaccessibility, antidiabetic activity and phenolic conversion of P. haitanensis were investigated at different harvests. Total polyphenol content (TPC), α-glucosidase inhibition and oxygen radical absorbance capacity (ORAC) increased with harvesting and digestion stages, reaching maximum at the fourth harvest. TPC and α-glucosidase inhibition after digestion reached 130-150 mg/g and 50-90 %, ORAC was 8.7-13.5 times higher than the undigestion. However, bioaccessibility in the first and second harvests was 10-80 % higher than other harvests. The phenolic content in the fourth harvest was up-regulated to 2-30 times than the first and mostly were citrus flavonoids. Redundancy analysis indicated significant correlation between phenolic metabolites and bioactivities in different harvests of P. haitanensis during digestion, with the strongest correlation coefficients were apigenin and genistein. This study provides reference for the application of P. haitanensis in treating type 2 diabetes.

摘要

紫菜的降血糖作用是众所周知的。基于体外消化和代谢组学，研究了不同采收期坛紫菜的生物可及性、抗糖尿病活性和酚类转化情况。总酚含量（TPC）、α-葡萄糖苷酶抑制率和氧自由基吸收能力（ORAC）随采收和消化阶段的增加而升高，在第四次采收时达到最大值。消化后的TPC和α-葡萄糖苷酶抑制率分别达到130 - 150mg/g和50 - 90%，ORAC比未消化时高8.7 - 13.5倍。然而，第一次和第二次采收的生物可及性比其他采收期高10 - 80%。第四次采收时的酚类含量比第一次上调了2 - 30倍，且大多为柑橘类黄酮。冗余分析表明，坛紫菜不同采收期消化过程中酚类代谢产物与生物活性之间存在显著相关性，其中芹菜素和染料木黄酮的相关系数最强。本研究为坛紫菜在治疗2型糖尿病中的应用提供了参考。