Agour Abdelkrim, Mssillou Ibrahim, Allali Aimad, Chebaibi Mohamed, El Abdali Youness, El Barnossi Azeddin, Bin Jardan Yousef A, Wondmie Gezahign Fentahun, Nafidi Hiba-Allah, Bourhia Mohammed, Bari Amina, Lyoussi Badiaa, Derwich Elhoussine
Laboratory of Natural Substances, Pharmacology, Environment, Modeling, Health, and Quality of Life, Faculty of Sciences Dhar El Mahraz, University Sidi Mohamed Ben Abdellah, Fez, Morocco.
Ministry of Health and Social Protection, Higher Institute of Nursing Professions and Health Techniques, Taza, Morocco.
Front Chem. 2023 Oct 6;11:1251449. doi: 10.3389/fchem.2023.1251449. eCollection 2023.
The present work aimed at characterizing the phytochemical composition of essential oil (HTEO), assessing its antifungal activity against various fungal strains, evaluating its insecticidal and repulsive properties against , and determine its antioxidant capacity. To this end, Gas chromatography-mass spectrometry analysis detected 34 compounds in HTEO, with -Caryophyllene being the major constituent (36.94%). HTEO demonstrated predominantly modest antifungal effects, however, it sustains notable activity, particularly against , with an inhibition rate of 76.50% ± 0.60%. Minimum inhibitory concentrations ranged from 20.53 ± 5.08 to 76.26 ± 5.08 mg/mL, effectively inhibiting fungal growth. Furthermore, the antifungal, and antioxidant activities of HTEO were evaluated against the proteins FAD glucose dehydrogenase, and beta-1,4-endoglucanase from , NAD(P)H Oxidase. Moreover, HTEO displayed strong insecticidal activity against , with contact and inhalation tests yielding LC values of 30.66 and 40.28 μL/100g, respectively, after 24 h of exposure. A dose of 5 μL/100g significantly reduced oviposition (48.85%) and inhibited emergence (45.15%) compared to the control group. Additionally, HTEO exhibited a high total antioxidant capacity of 758.34 mg AAE/g EO, highlighting its antioxidant potential. results showed that the antifungal activity of HTEO is mostly attributed to -Cadinene and p-Cymen-7-ol, while antioxidant is attributed to -Terpinyl isobutyrate displayed. Overall, HTEO offers a sustainable and environmentally friendly alternative to synthetic products used to manage diseases.
本研究旨在表征热罗勒精油（HTEO）的植物化学成分，评估其对各种真菌菌株的抗真菌活性，评价其对[具体昆虫名称未给出]的杀虫和驱避特性，并测定其抗氧化能力。为此，气相色谱 - 质谱分析在HTEO中检测到34种化合物，其中β-石竹烯是主要成分（36.94%）。HTEO主要表现出适度的抗真菌作用，然而，它具有显著活性，特别是对[具体真菌名称未给出]，抑制率为76.50%±0.60%。最低抑菌浓度范围为20.53±5.08至76.26±5.08mg/mL，有效抑制真菌生长。此外，还针对来自[具体微生物名称未给出]的蛋白质FAD葡萄糖脱氢酶、β-1,4-内切葡聚糖酶、NAD(P)H氧化酶评估了HTEO的抗真菌和抗氧化活性。此外，HTEO对[具体昆虫名称未给出]表现出较强的杀虫活性，接触和吸入试验在暴露24小时后，LC值分别为30.66和40.28μL/100g。与对照组相比，5μL/100g的剂量显著降低了产卵率（48.85%）并抑制了羽化率（45.15%）。此外，HTEO表现出758.34mg AAE/g EO的高总抗氧化能力，突出了其抗氧化潜力。结果表明，HTEO的抗真菌活性主要归因于α-杜松烯和对伞花烃 -7-醇，而抗氧化作用归因于所显示的异丁酸 - 萜品酯。总体而言，HTEO为用于疾病防治的合成产品提供了一种可持续且环保的替代品。
