Aimad Allali, Sanae Rezouki, Anas Fadli, Abdelfattah El Moussaoui, Bourhia Mohammed, Mohammad Salamatullah Ahmad, Alzahrani Abdulhakeem, Alyahya Heba Khalil, Albadr Nawal A, Abdelkrim Agour, El Barnossi Azeddin, Noureddine Eloutassi
Laboratory of Animal and Plant Production, and Agro-Industry, Faculty of Sciences, Ibn Tofail University BP 133, Kenitra 14000, Morocco.
Southwest Florida Research and Agricultural Center, IFAS, University of Florida, Immokalee, FL 34142, USA.
Evid Based Complement Alternat Med. 2021 Oct 15;2021:1108133. doi: 10.1155/2021/1108133. eCollection 2021.
The chemical composition and antibacterial, insecticidal, and antioxidant properties of the essential oil from L. () growing in Morocco were investigated in this work. To achieve this goal, the oils were obtained by using hydrodistillation before being characterized by GC-MS. The antibacterial and antifungal activities were conducted against pathogenic strains using the disc diffusion and MICS bioassays. The insecticidal activity was carried out versus using contact and inhalation tests. The antioxidant activity was performed by using DPPH and total antioxidant capacity bioassays. The chemical analysis of the oil showed that 20 compounds were identified, which represented 98.91% of the total oil. In the oil, the main components detected were R-(+)-pulegone (76.35%), carvone (5.84%), dihydrocarvone (5.09%), and octanol-3 (2.25%). The essential oil has moderate-to-strong broad-spectrum antibacterial and antifungal properties; the results showed that was the most sensitive strain to oil, with the largest inhibition diameter (25 ± 0.33). For the antifungal activity, the results obtained indicated that was the most sensitive fungal strain to oil with an inhibition percentage up to 100%. Regarding the insecticidal activity, the inhalation test showed a high efficacy (100% mortality), and a lethal concentration of LC = 1.41 + 0.48 L/L air was obtained after 24 hours of exposure. Moreover, the contact test showed that a total reduction in fertility and emergence was obtained with a dose of 20 L/mL of acetone. Regarding the antioxidant activity, the sample concentration necessary to inhibit 50% of HE radicals (IC) was 7.659 mg/mL (DPPH) and 583.066 57.05 mg EAA/g EO (TAC).
本研究对生长于摩洛哥的[植物名称]的精油的化学成分及其抗菌、杀虫和抗氧化特性进行了研究。为实现这一目标，采用水蒸馏法提取精油，然后用气相色谱 - 质谱联用仪（GC - MS）对其进行表征。使用纸片扩散法和最低抑菌浓度（MICS）生物测定法对病原菌进行抗菌和抗真菌活性测试。通过接触和吸入试验对[昆虫名称]进行杀虫活性测试。使用二苯基苦味酰基自由基（DPPH）和总抗氧化能力生物测定法进行抗氧化活性测试。精油的化学分析表明，共鉴定出20种化合物，占精油总量的98.91%。在该精油中，检测到的主要成分是R -（+）-胡薄荷酮（76.35%）、香芹酮（5.84%）、二氢香芹酮（5.09%）和3 - 辛醇（2.25%）。该精油具有中度至强的广谱抗菌和抗真菌特性；结果表明，[菌株名称]是对该精油最敏感的菌株，抑菌圈直径最大（25 ± 0.33）。对于抗真菌活性，所得结果表明，[真菌菌株名称]是对该精油最敏感的真菌菌株，抑制率高达100%。关于杀虫活性，吸入试验显示出高效（100%死亡率），暴露24小时后获得的致死浓度LC₅₀ = 1.41 ± 0.48 μL/L空气。此外，接触试验表明，使用20 μL/mL丙酮剂量可使繁殖力和羽化率完全降低。关于抗氧化活性，抑制50%羟自由基（IC₅₀）所需的样品浓度为7.659 mg/mL（DPPH）和583.066 ± 57.05 mg EAA/g EO（TAC）。
