The Forth Hospital of Hebei Medical University, Shijiazhuang, China.
Shenzhen Anxintang Biotechnology Co., Ltd, Shenzhen, China.
PeerJ. 2023 Oct 16;11:e16154. doi: 10.7717/peerj.16154. eCollection 2023.
To explore the specific protective mechanism of 3021 meal replacement powder (MRP) against non-alcoholic fatty liver disease (NAFLD).
C57BL/6J male mice were divided into four groups: control group, 3021 MRP group, model group and test group. The lipid accumulation and endoplasmic reticulum stress (ERS)-related proteins in hepatocytes of mice were detected by hematoxylin-eosin (HE) staining, oil red O staining and Western blotting.
The expressions of GRP78, GRP94, p-PERK and p-IRE1α were significantly inhibited in test group compared with those in model group. The protein expressions of p-NF-κB, p-JNK, IL-1β, IL-18 and NOX4 in test group were also significantly lower than those in model group. and experiments revealed that the body weight and lipid droplet content, and the expressions of ERS-related proteins (including BIP and XBP-1) in liver tissues all significantly declined in model group compared with those in 3021 MRP group.
In conclusion, 3021 MRP can greatly reduce lipid accumulation by inhibiting ERS, oxidative stress and inflammatory response in NAFLD.
探索 3021 代餐粉（MRP）防治非酒精性脂肪性肝病（NAFLD）的具体保护机制。
将雄性 C57BL/6J 小鼠分为 4 组：对照组、3021 MRP 组、模型组和试验组。采用苏木精-伊红（HE）染色、油红 O 染色和 Western blot 法检测各组小鼠肝细胞内脂质堆积和内质网应激（ERS）相关蛋白的表达情况。
与模型组相比，试验组 GRP78、GRP94、p-PERK 和 p-IRE1α 的表达均受到显著抑制。试验组 p-NF-κB、p-JNK、IL-1β、IL-18 和 NOX4 的蛋白表达也明显低于模型组。与 3021 MRP 组相比，模型组的体重和脂滴含量以及肝组织中 ERS 相关蛋白（包括 BIP 和 XBP-1）的表达均显著下降。
综上所述，3021 MRP 可通过抑制 ERS、氧化应激和炎症反应，显著减轻 NAFLD 中的脂质堆积。
