EGCG 的抗病毒活性、转录组学及其对感染. 的斑马鱼的保护作用。
The antivirulence activity, transcriptomics of EGCG and its protective effects on zebrafish infected by .
机构信息
School of Animal Science, Xichang University, Xichang, Sichuan, China.
Department of Pharmacy, Sichuan Agricultural University, Chengdu, China.
出版信息
Front Cell Infect Microbiol. 2023 Oct 6;13:1271448. doi: 10.3389/fcimb.2023.1271448. eCollection 2023.
BACKGROUND
is an important pathogen that mainly harms aquatic animals and exhibits resistance to a variety of antibiotics. This study investigated the effect of epigallocatechin-3-gallate (EGCG) on the virulence factors of and its impact on adhesion, invasion, and cytotoxicity in Caco-2 cells. The potential mechanism of antibacterial activity of EGCG was investigated by transcriptomic analysis.
RESULTS
EGCG not only inhibited the production of biofilm, hemolytic activity, motility, and protease activity of , but also reduced its adhesion, invasion, and cytotoxicity in Caco-2 cells. Transcriptomic analysis indicated that the antimicrobial activity of EGCG may be achieved by weakening the chemotaxis and stress response of the bacteria, as well as inhibiting the TonB system. Animal studies demonstrated that EGCG can significantly improve the survival rate and organs damage of zebrafish infected with .
CONCLUSION
EGCG would be a potential alternative drug for the prevention and treatment of infections by anti-virulence mechanism.
背景
是一种重要的病原体，主要危害水生动物，并对多种抗生素表现出耐药性。本研究探讨了表没食子儿茶素没食子酸酯（EGCG）对的毒力因子的影响及其对 Caco-2 细胞黏附、侵袭和细胞毒性的影响。通过转录组分析研究了 EGCG 的抗菌活性的潜在机制。
结果
EGCG 不仅抑制生物膜的产生、溶血活性、运动性和蛋白酶活性，还降低了其在 Caco-2 细胞中的黏附、侵袭和细胞毒性。转录组分析表明，EGCG 的抗菌活性可能是通过削弱细菌的趋化性和应激反应以及抑制 TonB 系统来实现的。动物研究表明，EGCG 可显著提高感染 的斑马鱼的存活率和器官损伤。
结论
EGCG 可能是一种通过抗毒力机制预防和治疗感染的潜在替代药物。
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